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PMID: 1188385 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

High speed scintillation autoradiography.

Science (New York, N.Y.) ·Vol. 190 ·No. 4219 ·1975-12-12 ·Pages 1093-5

Durie BG, Salmon SE

Abstract

Impregnation of nuclear track emulsion with liquid scintillator and exposure at -85 degrees C allows rapid autoradiographic labeling. With tritiated thymidine of high specific activity (40 to 60 curies per millimole), exposure time can be shortened to 20 to 60 minutes, allowing complete sample processing within 4 hours. In experiments requiring isotopes with low incorporation rates or low specific activity, exposure time can be shortened from months to several days.

MeSH Terms
Autoradiography/methods Bone Marrow/metabolism Lymphocytes/metabolism Myeloma Proteins/metabolism Scintillation Counting Temperature Thymidine/metabolism Time Factors
Chemicals
Myeloma Proteins Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Durie B G
Salmon S E
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
Published
1975-12-12
Pages
1093-5
Language
English
Region
United States
NLM ID
0404511
Subset
IM
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