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PMID: 1191264 Published · ppublish English Journal Article

The complexity of satellite deoxyribonucleic acid in a higher plant.

The Biochemical journal ·Vol. 149 ·No. 1 ·1975-07-00 ·Pages 31-8

Sinclair J, Wells R, Deumling B, Ingle J

Abstract

Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.

MeSH Terms
Base Sequence Centrifugation, Density Gradient Coliphages/analysis DNA DNA, Satellite/analysis DNA, Viral Hot Temperature Kinetics Nucleic Acid Denaturation Nucleic Acid Renaturation Plants/analysis Species Specificity
Chemicals
DNA, Satellite DNA, Viral DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sinclair J
Wells R
Deumling B
Ingle J
References (14)
14 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1490031
Published
1975-07-00
Pages
31-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165589
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1490031 PMC Full Text View journal details
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