Immune plasma-dependent cytotoxicity of immune and non-immune peripheral lymphoid cells for target cells coated with bacterial outer unit membrane.

Immunology ·Vol. 29 ·No. 6 ·1975-12-00 ·Pages 1093-102

Torten M, Johnson RC, Kaattari S, Leung C, Benjamini E

Abstract

The development of a model system for use in the study of lymphoid cell cytotoxicity to bacterial membrane antigens was attempted. In this system 51 Cr-labelled chicken red blood target cells were coated with pieces of the outer unit membrane of leptospirae rather than with soluble antigens. Using the model system to study dog peripheral immune lymphoid cell cytotoxicity to coated target cells we found that both immune and non-immune lymphocytes are antibody dependent for the expression of their cytotoxicity. It was also found that the unit membrane preparation from leptospirae can serve as a good antigenic stimulant to immune dog lymphoid cells as measured by increased [3H]thymidine uptake.

MeSH Terms

Animals Antigens, Bacterial Cell Membrane/immunology,ultrastructure Chickens/immunology Cytotoxicity Tests, Immunologic Dogs Erythrocytes/immunology,ultrastructure Hemagglutination Lectins Leptospira interrogans/immunology Lymphocytes/immunology,metabolism Thymidine/metabolism

Chemicals

Antigens, Bacterial Lectins Thymidine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Torten M

Johnson R C

Kaattari S

Leung C

Benjamini E

References (14)

14 references, click to expand

Cell-mediated cytotoxicity, allograft rejection, and tumor immunity.
Adv Immunol. 1974;18:67-132 PMID: 4151339
Cell-mediated immune response following Mycoplasma pneumoniae infection in man. I. Lymphocyte stimulation.
Clin Exp Immunol. 1974 May;17(1):29-41 PMID: 4466597
Cell-mediated immune response following Mycoplasma pneumoniae infection in man. II. Leucocyte migration inhibition.
Clin Exp Immunol. 1974 May;17(1):43-9 PMID: 4466598
Antibody-dependent cell-mediated cytotoxicity due to a "null" lymphoid cell.
Nat New Biol. 1973 Mar 28;242(117):111-3 PMID: 4541031
Antibody-mediated target cell lysis by nonimmune cells: the use of anti-immunoglobulin to distinguish effector cell population.
J Immunol. 1974 Feb;112(2):792-803 PMID: 4544363
Lymphoid cell-mediated antibody-dependent cytotoxicity in untreated and bursectomized chickens.
Clin Exp Immunol. 1974 Jan;16(1):137-40 PMID: 4549617
Isolation of the outer sheath of Leptospira and its immunogenic properties in hamsters.
Infect Immun. 1972 Jun;5(6):968-75 PMID: 4635506
Immunogenic properties of a leptospiral outer envelope bacterin in hamsters and foxes.
Am J Vet Res. 1974 May;35(5):681-4 PMID: 4830438
Serum induced lymphoid cell mediated cytotoxicity to human transitional cell carcinomas of the genitourinary tract.
Science. 1974 May 17;184(4138):795-7 PMID: 4856602
Lysis of chicken erythrocytes sensitized with PPD by lymphoid cells from guinea pigs immunized with tubercle bacilli.
Int Arch Allergy Appl Immunol. 1970;38(2):204-16 PMID: 4983711
Antibody-dependent lymphoid cell-mediated cytotoxicity: no requirement for thymus-derived lymphocytes.
Science. 1972 Jan 14;175(4018):194-6 PMID: 5008439
Simplified whole blood method for evaluating in vitro lymphocyte reactivity of laboratory animals.
Clin Exp Immunol. 1972 May;11(1):137-42 PMID: 5038770
Antibody-mediated target cell lysis by non-immune cells. Characterization of the antibody and effector cell populations.
Eur J Immunol. 1972 Oct;2(5):419-24 PMID: 5084393
Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes.
Cell Immunol. 1970 Sep;1(3):300-15 PMID: 5523583

Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1975-12-00

Pages

1093-102

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1446022

Subset

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Immune plasma-dependent cytotoxicity of immune and non-immune peripheral lymphoid cells for target cells coated with bacterial outer unit membrane.

Contact

WeChat Official Account