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PMID: 11943803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A developmental switch of AMPA receptor subunits in neocortical pyramidal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 22 ·No. 8 ·2002-04-15 ·Pages 3005-15

Kumar SS, Bacci A, Kharazia V, Huguenard JR

Abstract

AMPA receptors mediate most of the fast excitatory neurotransmission in the brain, and those lacking the glutamate receptor 2 (GluR2) subunit are Ca(2+)-permeable and expressed in cortical structures primarily by inhibitory interneurons. Here we report that synaptic AMPA receptors of excitatory layer 5 pyramidal neurons in the rat neocortex are deficient in GluR2 in early development, approximately before postnatal day 16, as evidenced by their inwardly rectifying current-voltage relationship, blockade of AMPA receptor-mediated EPSCs by external and internal polyamines, permeability to Ca(2+), and GluR2 immunoreactivity. Overall, these results indicate that neocortical pyramidal neurons undergo a developmental switch in the Ca(2+) permeability of their AMPA receptors through an alteration of their subunit composition. This has important implications for plasticity and neurotoxicity.

MeSH Terms
Aging/metabolism Animals Biogenic Polyamines/metabolism,pharmacology Calcium/metabolism Cell Membrane Permeability/physiology Electric Stimulation Excitatory Postsynaptic Potentials/drug effects,physiology In Vitro Techniques Neocortex/cytology,growth & development,metabolism Neuronal Plasticity/physiology Patch-Clamp Techniques Protein Subunits Pyramidal Cells/drug effects,metabolism Rats Rats, Sprague-Dawley Reaction Time/physiology Receptors, AMPA/deficiency,metabolism Synapses/metabolism Synaptic Transmission/physiology
Chemicals
Biogenic Polyamines Protein Subunits Receptors, AMPA glutamate receptor ionotropic, AMPA 2 Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kumar Sanjay S
Department of Neurology and Neurological Sciences, Stanford University Medical Center, Stanford, California 94305-5122, USA.
Bacci Alberto
Kharazia Viktor
Huguenard John R
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
20026285
Published
2002-04-15
Pages
3005-15
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6757523
Subset
IM
External Links
PubMed source DOI: 20026285 PMC Full Text View journal details
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