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PMID: 120161 Published · ppublish English Journal Article

The cell cycle of Bacillus subtilis as studied by electron microscopy.

Archives of microbiology ·Vol. 123 ·No. 2 ·1979-11-00 ·Pages 173-81

Nanninga N, Koppes LJ, de Vries-Tijssen FC

Abstract

Bacillus subtilis strain Marburg was grown exponentially with a doubling time of 65 min. To follow the time course of various cell cycle events, cells were collected by agar filtration and were then classified according to length. The DNA replication cycle was determined by a quantitative analysis of radioautograms of tritiated thymidine pulse labeled cells. The DNA replication period was found to be 45 min. This period is preceded and followed by periods without DNA synthesis of about 10 min. The morphology and segregation of nucleoplasmic bodies was studied in thin sections. B. subtilis contains two sets of genomes. DNA replication and DNA segregation seem to go hand in hand and DNA segregation is completed shortly after termination of DNA replication. Cell division and cell separation were investigated in whole mount preparations (agar filtration) and in thin sections. Cell division starts about 20 min after cell birth; cell separation starts at about 45 min and before completion of the septum.

MeSH Terms
Bacillus subtilis/cytology,metabolism,ultrastructure Cell Cycle Cell Division Cell Wall/ultrastructure DNA Replication DNA, Bacterial/biosynthesis
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nanninga N
Koppes L J
de Vries-Tijssen F C
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33 references, click to expand
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00446817
Published
1979-11-00
Pages
173-81
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00446817 View journal details
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