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PMID: 12019239 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Subfunctionalization of duplicate mitf genes associated with differential degeneration of alternative exons in fish.

Genetics ·Vol. 161 ·No. 1 ·2002-05-00 ·Pages 259-67

Altschmied J, Delfgaauw J, Wilde B, Duschl J, Bouneau L, Volff JN, Schartl M

Abstract

The microphthalmia-associated transcription factor (MITF) exists in at least four isoforms. These are generated in higher vertebrates using alternative 5' exons and promoters from a single gene. Two separate genes (mitf-m and mitf-b), however, are present in different teleost fish species including the poeciliid Xiphophorus, the pufferfishes Fugu rubripes and Tetraodon nigroviridis, and the zebrafish Danio rerio. Fish proteins MITF-m and MITF-b correspond at both the structural and the expression levels to one particular bird/mammalian MITF isoform. In the teleost lineage subfunctionalization of mitf genes after duplication at least 100 million years ago is associated with the degeneration of alternative exons and, probably, regulatory elements and promoters. For example, a remnant of the first exon specific for MITF-m is detected within the pufferfish gene encoding MITF-b. Retracing the evolutionary history of mitf genes in vertebrates uncovered the differential recruitment of new introns specific for either the teleost or the bird/mammalian lineage.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Cyprinodontiformes/genetics DNA-Binding Proteins/genetics Evolution, Molecular Exons Gene Duplication Microphthalmia-Associated Transcription Factor Molecular Sequence Data Phylogeny Sequence Alignment Tetraodontiformes/genetics Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Microphthalmia-Associated Transcription Factor Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Altschmied Joachim
Department of Physiological Chemistry I, Biocenter (Theodor-Boveri Institute), University of Würzburg, D-97074 Würzburg, Germany.
Delfgaauw Jacqueline
Wilde Brigitta
Duschl Jutta
Bouneau Laurence
Volff Jean-Nicolas
Schartl Manfred
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/161.1.259
Published
2002-05-00
Pages
259-67
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462118
Subset
IM
Databases
GENBANK
AF475090, AF475091
External Links
PubMed source DOI: 10.1093/genetics/161.1.259 PMC Full Text View journal details
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