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PMID: 12021331 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene.

Journal of virology ·Vol. 76 ·No. 12 ·2002-06-00 ·Pages 5985-92

Sugauchi F, Orito E, Ichida T, Kato H, Sakugawa H, Kakumu S, Ishida T, Chutaputti A, Lai CL, Ueda R, Miyakawa Y, Mizokami M

Abstract

The entire nucleotide sequences of 70 hepatitis B virus (HBV) isolates of genotype B (HBV/B), including 38 newly determined and 32 retrieved from the international DNA database (DDBJ/EMBL/GenBank), were compared phylogenetically. Two subgroups of HBV/B were identified based on sequence divergence in the precore region plus the core gene, one with the recombination with genotype C and the other without it. The analysis over the entire genome of HBV/B by the SimPlot program located the recombination with genotype C in the precore region plus the core gene spanning nucleotide positions from 1740 to 1838 to 2443 to 2485. Within this genomic area, HBV/B strains with the recombination had higher nucleotide and amino acid homology to genotype C than those without the recombination (96.9 versus 91.1% in nucleotides and 97.0 versus 92.9% in amino acids). There were 29 HBV/B strains without the recombination, and they were all recovered from carriers in Japan. The remaining 41 HBV/B isolates having the recombination with genotype C were from carriers in China (12 strains), Hong Kong (3 strains), Indonesia (4 strains), Japan (3 strains), Taiwan (4 strains), Thailand (3 strains), and Vietnam (12 strains). Due to the frequency of the distribution of HBV/B without the recombination (29 of 32 isolates, or 91%) and the fact that it was exclusive to Japan, it was provisionally classified into the Bj (j standing for Japan) subgroup, and HBV/B with the recombination was classified into the Ba (a for Asia) subgroup. Virological differences between HBV/Bj and HBV/Ba may be reflected in the severity of clinical disease in the patients infected with HBV of genotype B, which seems to be under strong geographic influences in Asia.

MeSH Terms
Base Sequence Genotype Hepatitis B virus/classification,genetics Humans Molecular Sequence Data Phylogeny Protein Precursors/genetics Recombination, Genetic Sequence Analysis, DNA Viral Core Proteins/genetics
Chemicals
Protein Precursors Viral Core Proteins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Sugauchi Fuminaka
Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Orito Etsuro
Ichida Takafumi
Kato Hideaki
Sakugawa Hiroshi
Kakumu Shinichi
Ishida Takafumi
Chutaputti Anuchit
Lai Ching-Ling
Ueda Ryuzo
Miyakawa Yuzo
Mizokami Masashi
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.12.5985-5992.2002
Published
2002-06-00
Pages
5985-92
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC136227
Subset
IM
Databases
GENBANK
AB073821, AB073822, AB073823, AB073824, AB073825, AB073826, AB073827, AB073828, AB073829, AB073830, AB073831, AB073832, AB073833, AB073834, AB073835, AB073836, AB073837, AB073838, AB073839, AB073840, AB073841, AB073842, AB073843, AB073844, AB073845, AB073846, AB073847, AB073848, AB073849, AB073850, AB073851, AB073852, AB073853, AB073854, AB073855, AB073856, AB073857, AB073858
External Links
PubMed source DOI: 10.1128/jvi.76.12.5985-5992.2002 PMC Full Text View journal details
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