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PMID: 12029035 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes.

Journal of bacteriology ·Vol. 184 ·No. 12 ·2002-06-00 ·Pages 3194-202

Murata T, Ohnishi M, Ara T, Kaneko J, Han CG, Li YF, Takashima K, Nojima H, Nakayama K, Kaji A, Kamio Y, Miki T, Mori H, Ohtsubo E, Terawaki Y, Hayashi T

Abstract

Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Chromosome Mapping Conjugation, Genetic DNA Replication DNA Transposable Elements Evolution, Molecular Genes, Duplicate Genome, Bacterial Molecular Sequence Data Open Reading Frames Plasmids/genetics Proteus vulgaris/genetics Replication Origin/genetics Sequence Analysis, DNA
Chemicals
Bacterial Proteins DNA Transposable Elements
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
Murata Takahiro
Department of Bacteriology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Ohnishi Makoto
Ara Takeshi
Kaneko Jun
Han Chang-Gyun
Li Yong Fang
Takashima Kayoko
Nojima Hideaki
Nakayama Keisuke
Kaji Akira
Kamio Yoshiyuki
Miki Takeyoshi
Mori Hirotada
Ohtsubo Eiichi
Terawaki Yoshiro
Hayashi Tetsuya
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.12.3194-3202.2002
Published
2002-06-00
Pages
3194-202
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135101
Subset
IM
Databases
GENBANK
AP004237
External Links
PubMed source DOI: 10.1128/JB.184.12.3194-3202.2002 PMC Full Text View journal details
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