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PMID: 12032338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 11 ·2002-05-28 ·Pages 7652-7

Petnicki-Ocwieja T, Schneider DJ, Tam VC, Chancey ST, Shan L, Jamir Y, Schechter LM, Janes MD, Buell CR, Tang X, Collmer A, Alfano JR

Abstract

The ability of Pseudomonas syringae pv. tomato DC3000 to be pathogenic on plants depends on the Hrp (hypersensitive response and pathogenicity) type III protein secretion system and the effector proteins it translocates into plant cells. Through iterative application of experimental and computational techniques, the DC3000 effector inventory has been substantially enlarged. Five homologs of known avirulence (Avr) proteins and five effector candidates, encoded by genes with putative Hrp promoters and signatures of horizontal acquisition, were demonstrated to be secreted in culture and/or translocated into Arabidopsis in a Hrp-dependent manner. These 10 Hrp-dependent outer proteins (Hops) were designated HopPtoC (AvrPpiC2 homolog), HopPtoD1 and HopPtoD2 (AvrPphD homologs), HopPtoK (AvrRps4 homolog), HopPtoJ (AvrXv3 homolog), HopPtoE, HopPtoG, HopPtoH, HopPtoI, and HopPtoS1 (an ADP-ribosyltransferase homolog). Analysis of the enlarged collection of proteins traveling the Hrp pathway in P. syringae revealed an export-associated pattern of equivalent solvent-exposed amino acids in the N-terminal five positions, a lack of Asp or Glu residues in the first 12 positions, and amphipathicity in the first 50 positions. These characteristics were used to search the unfinished DC3000 genome, yielding 32 additional candidate effector genes that predicted proteins with Hrp export signals and that also possessed signatures of horizontal acquisition. Among these were genes encoding additional ADP-ribosyltransferases, a homolog of SrfC (a candidate effector in Salmonella enterica), a catalase, and a glucokinase. One ADP-ribosyltransferase and the SrfC homolog were tested and shown to be secreted in a Hrp-dependent manner. These proteins, designated HopPtoS2 and HopPtoL, respectively, bring the DC3000 Hrp-secreted protein inventory to 22.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,microbiology Bacterial Proteins/genetics,metabolism Cloning, Molecular Escherichia coli/genetics Genome, Bacterial Molecular Sequence Data Open Reading Frames Polymerase Chain Reaction Pseudomonas/genetics,pathogenicity Sequence Alignment Sequence Homology, Amino Acid Translocation, Genetic Virulence/genetics
Chemicals
Bacterial Proteins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Petnicki-Ocwieja Tanja
Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588-0660, USA.
Schneider David J
Tam Vincent C
Chancey Scott T
Shan Libo
Jamir Yashitola
Schechter Lisa M
Janes Misty D
Buell C Robin
Tang Xiaoyan
Collmer Alan
Alfano James R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.112183899
Published
2002-05-28
Pages
7652-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC124312
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.112183899 PMC Full Text View journal details
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