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PMID: 12034773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Rho-associated protein kinase p160ROCK is required for centrosome positioning.

The Journal of cell biology ·Vol. 157 ·No. 5 ·2002-05-27 ·Pages 807-17

Chevrier V, Piel M, Collomb N, Saoudi Y, Frank R, Paintrand M, Narumiya S, Bornens M, Job D

Abstract

The p160-Rho-associated coiled-coil-containing protein kinase (ROCK) is identified as a new centrosomal component. Using immunofluorescence with a variety of p160ROCK antibodies, immuno EM, and depletion with RNA interference, p160ROCK is principally bound to the mother centriole (MC) and an intercentriolar linker. Inhibition of p160ROCK provoked centrosome splitting in G1 with the MC, which is normally positioned at the cell center and shows little motion during G1, displaying wide excursions around the cell periphery, similar to its migration toward the midbody during cytokinesis. p160ROCK inhibition late after anaphase in mitosis triggered MC migration to the midbody followed by completion of cell division. Thus, p160ROCK is required for centrosome positioning and centrosome-dependent exit from mitosis.

MeSH Terms
Anaphase/physiology Animals Antibodies Cattle Centrioles/metabolism,ultrastructure Cloning, Molecular G1 Phase/physiology HeLa Cells Humans Intracellular Signaling Peptides and Proteins Microscopy, Immunoelectron Protein Serine-Threonine Kinases/genetics,immunology,metabolism Rabbits rho-Associated Kinases
Chemicals
Antibodies Intracellular Signaling Peptides and Proteins Protein Serine-Threonine Kinases rho-Associated Kinases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Chevrier Véronique
Institut National de la Santé et de la Recherche Medicale U366, Département de Biologie Moléculaire et Structurale/Cytosqulette, Commissariat à l'Energie Atomique, de Grenoble, 38054 Grenoble Cedex 9, France.
Piel Matthieu
Collomb Nora
Saoudi Yasmina
Frank Ronald
Paintrand Michel
Narumiya Shuh
Bornens Michel
Job Didier
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200203034
Published
2002-05-27
Epub
2002-00-28
Pages
807-17
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173415
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200203034 PMC Full Text View journal details
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