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PMID: 12055303 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic diversity of three lgt loci for biosynthesis of lipooligosaccharide (LOS) in Neisseria species.

Microbiology (Reading, England) ·Vol. 148 ·No. Pt 6 ·2002-06-00 ·Pages 1833-1844

Zhu P, Klutch MJ, Bash MC, Tsang RSW, Ng LK, Tsai CM

Abstract

Lipooligosaccharide (LOS) is a major virulence factor of the pathogenic Neisseria. Nine lgt genes at three chromosomal loci (lgt-1, 2, 3) encoding the glycosyltransferases responsible for the biosynthesis of LOS oligosaccharide chains were examined in 26 Neisseria meningitidis, 51 Neisseria gonorrhoeae and 18 commensal Neisseria strains. DNA hybridization, PCR and nucleotide sequence data were compared to previously reported lgt genes. Analysis of the genetic organization of the lgt loci revealed that in N. meningitidis, the lgt-1 and lgt-3 loci were hypervariable genomic regions, whereas the lgt-2 locus was conserved. In N. gonorrhoeae, no variability in the composition or organization of the three lgt loci was observed. lgt genes were detected only in some commensal Neisseria species. The genetic organization of the lgt-1 locus was classified into eight types and the lgt-3 locus was classified into four types. Two types of arrangement at lgt-1 (II and IV) and one type of arrangement at lgt-3 (IV) were novel genetic organizations reported in this study. Based on the three lgt loci, 10 LOS genotypes of N. meningitidis were distinguished. Phylogenetic analysis revealed a gene cluster, lgtH, which separated from the homologous genes lgtB and lgtE. The lgtH and lgtE genes were mutually exclusive and were located at the same position in lgt-1. The data demonstrated that pathogenic and commensal Neisseria share a common lgt gene pool and horizontal gene transfer appears to contribute to the genetic diversity of the lgt loci in Neisseria.

MeSH Terms
Genes, Bacterial/genetics Genetic Variation/genetics Genotype Glycosyltransferases/genetics Lipopolysaccharides/biosynthesis Molecular Sequence Data N-Acetylglucosaminyltransferases/genetics Neisseria meningitidis/classification,genetics,growth & development,metabolism Phylogeny Polymorphism, Genetic/genetics Sequence Analysis, DNA
Chemicals
Lipopolysaccharides lipid-linked oligosaccharides Glycosyltransferases N-Acetylglucosaminyltransferases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhu Peixuan
Division of Bacterial, Parasitic and Allergenic Products1 and Division of Viral Products2, Center for Biologics Evaluation and Research, FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Klutch Michael J
Division of Bacterial, Parasitic and Allergenic Products1 and Division of Viral Products2, Center for Biologics Evaluation and Research, FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Bash Margaret C
Division of Bacterial, Parasitic and Allergenic Products1 and Division of Viral Products2, Center for Biologics Evaluation and Research, FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Tsang Raymond S W
National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Canada3.
Ng Lai-King
National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Canada3.
Tsai Chao-Ming
Division of Bacterial, Parasitic and Allergenic Products1 and Division of Viral Products2, Center for Biologics Evaluation and Research, FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Article Info
Journal
Microbiology (Reading, England)
Abbr.
Microbiology (Reading)
ISSN
1350-0872
DOI
10.1099/00221287-148-6-1833
Published
2002-06-00
Pages
1833-1844
Language
English
Region
England
NLM ID
9430468
Subset
IM
Grants
FDA HHS · 369VFFD018551 · United States
Databases
GENBANK
AF470655, AF470656, AF470657, AF470658, AF470659, AF470660, AF470661, AF470662, AF470663, AF470664, AF470665, AF470666, AF470667, AF470668, AF470669, AF470670, AF470671, AF470672, AF470673, AF470674, AF470675, AF470676, AF470677, AF470678, AF470679, AF470680, AF470681, AF470682, AF470683, AF470684, AF470685
External Links
PubMed source DOI: 10.1099/00221287-148-6-1833 View journal details
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