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PMID: 12057934 Published · ppublish English Journal Article

Molecular characterization of the PceA reductive dehalogenase of desulfitobacterium sp. strain Y51.

Journal of bacteriology ·Vol. 184 ·No. 13 ·2002-07-00 ·Pages 3419-25

Suyama A, Yamashita M, Yoshino S, Furukawa K

Abstract

The tetrachloroethene (PCE) reductive dehalogenase (encoded by the pceA gene and designated PceA dehalogenase) of Desulfitobacterium sp. strain Y51 was purified and characterized. The expression of the enzyme was highly induced in the presence of PCE and trichloroethene (TCE). The purified enzyme catalyzed the reductive dehalogenation of PCE via TCE to cis-1,2-dichloroethene at a specific activity of 113.6 nmol x min(-1) x mg of protein(-1). The apparent K(m) values for PCE and TCE were 105.7 and 535.3 microM, respectively. Chlorinated ethenes other than PCE and TCE were not dehalogenated. However, the enzyme exhibited dehalogenation activity for various chlorinated ethanes such as hexachloroethane, pentachloroethane, 1,1,1,2-tetrachloroethane, and 1,1,2,2-tetrachloroethane. The pceA gene of Desulfitobacterium sp. strain Y51 was identified in a 2.8-kb DNA fragment and used to express the protein in Escherichia coli for the preparation of antibodies. Immunoblot analyses located PceA in the periplasm of the cell.

MeSH Terms
Amino Acid Sequence Cloning, Molecular Cross Reactions Electrophoresis, Polyacrylamide Gel Enzyme Activation Escherichia coli/genetics Gram-Positive Bacteria/enzymology,genetics Molecular Sequence Data Oxidoreductases/genetics,immunology,metabolism Sequence Analysis Sequence Homology, Amino Acid
Chemicals
Oxidoreductases tetrachloroethene dehalogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Suyama Akiko
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581. Towakagaku Co., Ltd., Hiroshima 730-0841, Japan.
Yamashita Masaki
Yoshino Sadazo
Furukawa Kensuke
References (29)
29 references, click to expand
  1. The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus".
    J Bacteriol. 1996 Apr;178(8):2328-33 PMID: 8636034
  2. Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway.
    Appl Environ Microbiol. 1998 Apr;64(4):1270-5 PMID: 10671186
  3. Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans.
    J Biol Chem. 1996 Jul 12;271(28):16515-9 PMID: 8663199
  4. Tetrachloroethene-dehalogenating bacteria.
    Folia Microbiol (Praha). 1999;44(3):247-62 PMID: 10664879
  5. Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum. Homologies with other cobalamin-dependent enzymes.
    FEBS Lett. 1992 Sep 28;310(2):167-70 PMID: 1397267
  6. Reductive dechlorination of chlorinated ethenes and 1, 2-dichloroethane by "Dehalococcoides ethenogenes" 195.
    Appl Environ Microbiol. 1999 Jul;65(7):3108-13 PMID: 10388710
  7. Overlapping functions of components of a bacterial Sec-independent protein export pathway.
    EMBO J. 1998 Jul 1;17(13):3640-50 PMID: 9649434
  8. Structure, function and evolution of bacterial ferredoxins.
    FEMS Microbiol Rev. 1988 Apr-Jun;4(2):155-75 PMID: 3078742
  9. Reductive dehalogenation as a respiratory process.
    Antonie Van Leeuwenhoek. 1994;66(1-3):239-46 PMID: 7747935
  10. In vitro dehalogenation of tetrachloroethylene (PCE) by cell-free extracts of Clostridium bifermentans DPH-1.
    Bioresour Technol. 2001 Jun;78(2):141-7 PMID: 11333032
  11. Isolation and characterization of Desulfitobacterium sp. strain Y51 capable of efficient dehalogenation of tetrachloroethene and polychloroethanes.
    Biosci Biotechnol Biochem. 2001 Jul;65(7):1474-81 PMID: 11515528
  12. Purification, cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethylene (PCE) from Clostridium bifermentans DPH-1.
    Can J Microbiol. 2001 May;47(5):448-56 PMID: 11400736
  13. Tetrachloroethene metabolism of Dehalospirillum multivorans.
    Arch Microbiol. 1994;162(4):295-301 PMID: 7802545
  14. Anaerobic dehalogenases.
    Curr Opin Biotechnol. 1997 Jun;8(3):290-5 PMID: 9206009
  15. Purification and characterization of the tetrachloroethene reductive dehalogenase of strain PCE-S.
    Arch Microbiol. 1998 Jun;169(6):497-502 PMID: 9575235
  16. Dehalobacter restrictus gen. nov. and sp. nov., a strictly anaerobic bacterium that reductively dechlorinates tetra- and trichloroethene in an anaerobic respiration.
    Arch Microbiol. 1998 Apr;169(4):313-21 PMID: 9531632
  17. Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli.
    J Bacteriol. 1998 Aug;180(16):4140-5 PMID: 9696761
  18. Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene.
    Science. 1997 Jun 6;276(5318):1568-71 PMID: 9171062
  19. Trichloroethene reductive dehalogenase from Dehalococcoides ethenogenes: sequence of tceA and substrate range characterization.
    Appl Environ Microbiol. 2000 Dec;66(12):5141-7 PMID: 11097881
  20. Purification and molecular characterization of ortho-chlorophenol reductive dehalogenase, a key enzyme of halorespiration in Desulfitobacterium dehalogenans.
    J Biol Chem. 1999 Jul 16;274(29):20287-92 PMID: 10400648
  21. Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S.
    Arch Microbiol. 1997 Dec;168(6):513-9 PMID: 9385143
  22. Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.
    Microbiol Rev. 1994 Dec;58(4):641-85 PMID: 7854251
  23. Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and outer membranes of donor cells: implications for related macromolecule transport systems.
    J Bacteriol. 2000 Mar;182(6):1564-74 PMID: 10692361
  24. Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus.
    FEBS Lett. 1997 Jun 16;409(3):421-5 PMID: 9224702
  25. Studies on tetrachloroethene respiration in Dehalospirillum multivorans.
    Arch Microbiol. 1996 Dec;166(6):379-87 PMID: 9082914
  26. Transcriptional regulation of the cpr gene cluster in ortho-chlorophenol-respiring Desulfitobacterium dehalogenans.
    J Bacteriol. 2000 Oct;182(20):5683-91 PMID: 11004165
  27. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  28. The Tat protein export pathway.
    Mol Microbiol. 2000 Jan;35(2):260-74 PMID: 10652088
  29. A common export pathway for proteins binding complex redox cofactors?
    Mol Microbiol. 1996 Nov;22(3):393-404 PMID: 8939424
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.13.3419-3425.2002
Published
2002-07-00
Pages
3419-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135124
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.184.13.3419-3425.2002 PMC Full Text View journal details
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