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PMID: 12084916 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 13 ·2002-06-25 ·Pages 8548-53

Peng G, Hopper JE

Abstract

Galactose-inducible genes (GAL genes) in yeast Saccharomyces cerevisiae are efficiently transcribed only when the sequence-specific transcription activator Gal4p is activated. Activation of Gal4p requires the interaction between the Gal4p inhibitory protein Gal80p and the galactokinase paralog, Gal3p. It has been proposed that Gal3p binds to a Gal80p-Gal4p complex in the nucleus to activate Gal4p. Here, we present evidence that the Gal3p-Gal80p interaction occurs in the cytoplasm, and concurrently, Gal80p is removed from Gal4p at the GAL gene promoter. We also show that GAL gene expression can be activated by heterologous protein-protein interaction in the cytoplasm that is independent of galactose and Gal3p function. These results indicate that galactose-triggered Gal3p-Gal80p association in the cytoplasm activates Gal4p in the nucleus.

MeSH Terms
Base Sequence Cytoplasm/metabolism DNA Primers Fungal Proteins/metabolism Gene Expression Regulation Microscopy, Fluorescence Precipitin Tests Promoter Regions, Genetic Repressor Proteins Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/metabolism Signal Transduction Transcription Factors/metabolism Transcriptional Activation
Chemicals
DNA Primers Fungal Proteins GAL80 protein, S cerevisiae Gal3 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peng Gang
Graduate Program in Biochemistry and Molecular Biology, Intercollege Graduate Program in Genetics, and Department of Biochemistry and Molecular Biology, Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA.
Hopper James E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.142100099
Published
2002-06-25
Pages
8548-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC124307
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.142100099 PMC Full Text View journal details
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