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PMID: 12093871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chemokine requirements for B cell entry to lymph nodes and Peyer's patches.

The Journal of experimental medicine ·Vol. 196 ·No. 1 ·2002-07-01 ·Pages 65-75

Okada T, Ngo VN, Ekland EH, Förster R, Lipp M, Littman DR, Cyster JG

Abstract

B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4-/- B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.

MeSH Terms
Animals B-Lymphocytes/cytology,immunology,metabolism Cell Adhesion/immunology Cell Movement/immunology Chemokine CXCL12 Chemokine CXCL13 Chemokines/metabolism Chemokines, CXC/genetics,metabolism Endothelium, Vascular/cytology,immunology,metabolism Lymph Nodes/cytology,immunology Mice Mice, Inbred Strains Mice, Mutant Strains Peyer's Patches/cytology,immunology RNA, Messenger/metabolism Receptors, CCR7 Receptors, CXCR4/antagonists & inhibitors,metabolism Receptors, CXCR5 Receptors, Chemokine/antagonists & inhibitors,metabolism Receptors, Cytokine/metabolism Receptors, Lymphocyte Homing/metabolism Signal Transduction/physiology Venules
Chemicals
CXCR5 protein, mouse Ccr7 protein, mouse Chemokine CXCL12 Chemokine CXCL13 Chemokines Chemokines, CXC Cxcl12 protein, mouse Cxcl13 protein, mouse RNA, Messenger Receptors, CCR7 Receptors, CXCR4 Receptors, CXCR5 Receptors, Chemokine Receptors, Cytokine Receptors, Lymphocyte Homing
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Okada Takaharu
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA.
Ngo Vu N
Ekland Eric H
Förster Reinhold
Lipp Martin
Littman Dan R
Cyster Jason G
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20020201
Published
2002-07-01
Pages
65-75
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2194009
Subset
IM
Grants
NIAID NIH HHS · R01 AI045073 · United States
NIAID NIH HHS · AI45073 · United States
External Links
PubMed source DOI: 10.1084/jem.20020201 PMC Full Text View journal details
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