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PMID: 12096187 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 15 ·2002-07-23 ·Pages 9948-53

Huet F, Lu JT, Myrick KV, Baugh LR, Crosby MA, Gelbart WM

Abstract

With the available eukaryotic genome sequences, there are predictions of thousands of previously uncharacterized genes without known function or available mutational variant. Thus, there is an urgent need for efficient genetic tools for genomewide phenotypic analysis. Here we describe such a tool: a deletion-generator technology that exploits properties of a double transposable element to produce molecularly defined deletions at high density and with high efficiency. This double element, called P[wHy], is composed of a "deleter" element hobo, bracketed by two genetic markers and inserted into a "carrier" P element. We have used this P[wHy] element in Drosophila melanogaster to generate sets of nested deletions of sufficient coverage to discriminate among every transcription unit within 60 kb of the starting insertion site. Because these two types of mobile elements, carrier and deleter, can be found in other species, our strategy should be applicable to phenotypic analysis in a variety of model organisms.

MeSH Terms
Animals Chromosome Mapping DNA Replication Drosophila melanogaster/genetics Gene Deletion Genome Molecular Sequence Data Phenotype Sequence Deletion
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Huet François
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
Lu Jeffrey T
Myrick Kyl V
Baugh L Ryan
Crosby Madeline A
Gelbart William M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.142310099
Published
2002-07-23
Epub
2002-00-02
Pages
9948-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC126605
Subset
IM
Grants
NIGMS NIH HHS · R37 GM028669 · United States
NIGMS NIH HHS · 5 R37 GM28669 · United States
Databases
GENBANK
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Corrections
CommentIn
PMID: 12122216
External Links
PubMed source DOI: 10.1073/pnas.142310099 PMC Full Text View journal details
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