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PMID: 12097649 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Intercellular calcium signaling mediated by point-source burst release of ATP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 15 ·2002-07-23 ·Pages 9840-5

Arcuino G, Lin JH, Takano T, Liu C, Jiang L, Gao Q, Kang J, Nedergaard M

Abstract

Calcium signaling, manifested as intercellular waves of rising cytosolic calcium, is, in many cell types, the result of calcium-induced secretion of ATP and activation of purinergic receptors. The mechanism by which ATP is released has hitherto not been established. Here, we show by real-time bioluminescence imaging that ATP efflux is not uniform across a field of cells but is restricted to brief, abrupt point-source bursts. The ATP bursts emanate from single cells and manifest the transient opening of nonselective membrane channels, which admits fluorescent indicators of < or = 1.5 kDa. These observations challenge the existence of regenerative ATP release, because ATP efflux is finite and restricted to a point source. Transient efflux of cytosolic nucleotides from a subset of cells may represent a conserved pathway for coordinating local activity of electrically nonexcitable cells, because identical patterns of ATP release were identified in human astrocytes, endothelial cells, and bronchial epithelial cells.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Animals, Newborn Astrocytes/cytology,physiology Calcium Signaling/physiology Cell Communication/physiology Cells, Cultured Cerebral Cortex/cytology Endothelium, Vascular/physiology Humans Kinetics Rats Respiratory Mucosa/cytology,physiology Umbilical Veins
Chemicals
Adenosine Triphosphate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Arcuino Gregory
Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595, USA.
Lin Jane H-C
Takano Takahiro
Liu Collins
Jiang Li
Gao Qun
Kang Jian
Nedergaard Maiken
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.152588599
Published
2002-07-23
Epub
2002-00-03
Pages
9840-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC125036
Subset
IM
Grants
NINDS NIH HHS · R01 NS030007 · United States
NINDS NIH HHS · NS37349 · United States
NINDS NIH HHS · R01 NS038073 · United States
NINDS NIH HHS · NS30007 · United States
NINDS NIH HHS · NS38073 · United States
External Links
PubMed source DOI: 10.1073/pnas.152588599 PMC Full Text View journal details
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