Home LiteratureArticle Details
PMID: 12101121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MicroRNAs in plants.

Genes & development ·Vol. 16 ·No. 13 ·2002-07-01 ·Pages 1616-26

Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP

Abstract

MicroRNAs (miRNAs) are an extensive class of ~22-nucleotide noncoding RNAs thought to regulate gene expression in metazoans. We find that miRNAs are also present in plants, indicating that this class of noncoding RNA arose early in eukaryotic evolution. In this paper 16 Arabidopsis miRNAs are described, many of which have differential expression patterns in development. Eight are absolutely conserved in the rice genome. The plant miRNA loci potentially encode stem-loop precursors similar to those processed by Dicer (a ribonuclease III) in animals. Mutation of an Arabidopsis Dicer homolog, CARPEL FACTORY, prevents the accumulation of miRNAs, showing that similar mechanisms direct miRNA processing in plants and animals. The previously described roles of CARPEL FACTORY in the development of Arabidopsis embryos, leaves, and floral meristems suggest that the miRNAs could play regulatory roles in the development of plants as well as animals.

MeSH Terms
Base Sequence Evolution, Molecular MicroRNAs Molecular Sequence Data Nucleic Acid Conformation Plant Development Plants/genetics RNA, Antisense/chemistry,genetics RNA, Plant/chemistry,genetics RNA, Untranslated/chemistry,genetics
Chemicals
MicroRNAs RNA, Antisense RNA, Plant RNA, Untranslated
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reinhart Brenda J
Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Weinstein Earl G
Rhoades Matthew W
Bartel Bonnie
Bartel David P
References (50)
50 references, click to expand
  1. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA.
    Science. 2001 Aug 3;293(5531):834-8 PMID: 11452083
  2. The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene.
    Development. 1999 Feb;126(3):469-81 PMID: 9876176
  3. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
    Nature. 1998 Feb 19;391(6669):806-11 PMID: 9486653
  4. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans.
    Genes Dev. 2001 Oct 15;15(20):2654-9 PMID: 11641272
  5. The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes.
    Plant J. 1999 Apr;18(1):111-9 PMID: 10341448
  6. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells.
    Nature. 2000 Mar 16;404(6775):293-6 PMID: 10749213
  7. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.
    Cell. 2001 Jul 13;106(1):23-34 PMID: 11461699
  8. RNAi: nature abhors a double-strand.
    Curr Opin Genet Dev. 2002 Apr;12(2):225-32 PMID: 11893497
  9. Identification of tissue-specific microRNAs from mouse.
    Curr Biol. 2002 Apr 30;12(9):735-9 PMID: 12007417
  10. DNA methylation and chromatin structure affect transcriptional and post-transcriptional transgene silencing in Arabidopsis.
    Curr Biol. 2000 Dec 14-28;10(24):1591-4 PMID: 11137011
  11. A genetic link between co-suppression and RNA interference in C. elegans.
    Nature. 2000 Mar 16;404(6775):296-8 PMID: 10749214
  12. The rde-1 gene, RNA interference, and transposon silencing in C. elegans.
    Cell. 1999 Oct 15;99(2):123-32 PMID: 10535731
  13. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.
    Science. 2001 Oct 26;294(5543):858-62 PMID: 11679671
  14. An extensive class of small RNAs in Caenorhabditis elegans.
    Science. 2001 Oct 26;294(5543):862-4 PMID: 11679672
  15. Argonaute2, a link between genetic and biochemical analyses of RNAi.
    Science. 2001 Aug 10;293(5532):1146-50 PMID: 11498593
  16. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.
    Cell. 2000 Mar 31;101(1):25-33 PMID: 10778853
  17. Maternal effects of the short integument mutation on embryo development in Arabidopsis.
    Dev Biol. 1996 Nov 25;180(1):365-9 PMID: 8948599
  18. AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11650-4 PMID: 11016954
  19. Female sterile mutations on the second chromosome of Drosophila melanogaster. II. Mutations blocking oogenesis or altering egg morphology.
    Genetics. 1991 Dec;129(4):1119-36 PMID: 1783295
  20. ATP requirements and small interfering RNA structure in the RNA interference pathway.
    Cell. 2001 Nov 2;107(3):309-21 PMID: 11701122
  21. Ovule Development in Wild-Type Arabidopsis and Two Female-Sterile Mutants.
    Plant Cell. 1992 Oct;4(10):1237-1249 PMID: 12297633
  22. Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems.
    Development. 1999 Dec;126(23):5231-43 PMID: 10556049
  23. A draft sequence of the rice genome (Oryza sativa L. ssp. indica).
    Science. 2002 Apr 5;296(5565):79-92 PMID: 11935017
  24. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.
    Nature. 2000 Feb 24;403(6772):901-6 PMID: 10706289
  25. Fertile hypomorphic ARGONAUTE (ago1) mutants impaired in post-transcriptional gene silencing and virus resistance.
    Plant Cell. 2002 Mar;14(3):629-39 PMID: 11910010
  26. ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos.
    Proc Natl Acad Sci U S A. 2002 May 14;99(10):6889-94 PMID: 12011447
  27. RNA silencing in plants--defense and counterdefense.
    Science. 2001 Jun 22;292(5525):2277-80 PMID: 11423650
  28. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
    Cell. 1993 Dec 3;75(5):843-54 PMID: 8252621
  29. Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline.
    Curr Biol. 2001 Jul 10;11(13):1017-27 PMID: 11470406
  30. Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation.
    Nat Genet. 2002 Apr;30(4):363-4 PMID: 11896390
  31. Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance.
    Cell. 2000 May 26;101(5):533-42 PMID: 10850495
  32. miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.
    Genes Dev. 2002 Mar 15;16(6):720-8 PMID: 11914277
  33. An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus.
    Cell. 2000 May 26;101(5):543-53 PMID: 10850496
  34. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans.
    Cell. 1993 Dec 3;75(5):855-62 PMID: 8252622
  35. A species of small antisense RNA in posttranscriptional gene silencing in plants.
    Science. 1999 Oct 29;286(5441):950-2 PMID: 10542148
  36. Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis.
    EMBO J. 1998 Mar 16;17(6):1799-809 PMID: 9501101
  37. SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis.
    EMBO J. 2001 Apr 17;20(8):2069-78 PMID: 11296239
  38. Domains in gene silencing and cell differentiation proteins: the novel PAZ domain and redefinition of the Piwi domain.
    Trends Biochem Sci. 2000 Oct;25(10):481-2 PMID: 11050429
  39. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  40. Identification of novel genes coding for small expressed RNAs.
    Science. 2001 Oct 26;294(5543):853-8 PMID: 11679670
  41. The lin-41 RBCC gene acts in the C. elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor.
    Mol Cell. 2000 Apr;5(4):659-69 PMID: 10882102
  42. Non-coding RNA genes and the modern RNA world.
    Nat Rev Genet. 2001 Dec;2(12):919-29 PMID: 11733745
  43. RNA-based silencing strategies in plants.
    Curr Opin Genet Dev. 2001 Apr;11(2):221-7 PMID: 11250148
  44. SHORT INTEGUMENT (SIN1), a gene required for ovule development in Arabidopsis, also controls flowering time.
    Development. 1996 Sep;122(9):2631-8 PMID: 8787738
  45. Role for a bidentate ribonuclease in the initiation step of RNA interference.
    Nature. 2001 Jan 18;409(6818):363-6 PMID: 11201747
  46. AGO1 defines a novel locus of Arabidopsis controlling leaf development.
    EMBO J. 1998 Jan 2;17(1):170-80 PMID: 9427751
  47. The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root.
    Cell. 1996 Aug 9;86(3):423-33 PMID: 8756724
  48. Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA.
    Nature. 2000 Nov 2;408(6808):86-9 PMID: 11081512
  49. RNA interference is mediated by 21- and 22-nucleotide RNAs.
    Genes Dev. 2001 Jan 15;15(2):188-200 PMID: 11157775
  50. A vicious cycle: RNA silencing and DNA methylation in plants.
    Cell. 2001 Jul 27;106(2):129-32 PMID: 11511339
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1004402
Published
2002-07-01
Pages
1616-26
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC186362
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1101/gad.1004402 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2