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PMID: 12121960 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Extrusion of penem antibiotics by multicomponent efflux systems MexAB-OprM, MexCD-OprJ, and MexXY-OprM of Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy ·Vol. 46 ·No. 8 ·2002-08-00 ·Pages 2696-9

Okamoto K, Gotoh N, Nishino T

Abstract

The high intrinsic penem resistance of Pseudomonas aeruginosa is due to the interplay among the outer membrane barrier, the active efflux system MexAB-OprM, and AmpC beta-lactamase. We studied the roles of two other efflux systems, MexCD-OprJ and MexXY-OprM, in penem resistance by overexpressing each system in an AmpC- and MexAB-OprM-deficient background and found that MexAB-OprM is the most important among the three efflux systems for extrusion of penems from the cell interior.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/genetics Carbapenems/metabolism Carrier Proteins/genetics Drug Resistance, Microbial Genome, Bacterial Immunoblotting Membrane Proteins/genetics Membrane Transport Proteins/genetics Microbial Sensitivity Tests Mutation/genetics Plasmids/genetics Pseudomonas aeruginosa/drug effects,genetics beta-Lactamases/metabolism
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Carbapenems Carrier Proteins Membrane Proteins Membrane Transport Proteins MexA protein, Pseudomonas aeruginosa MexB protein, Pseudomonas aeruginosa MexC protein, Pseudomonas aeruginosa MexD protein, Pseudomonas aeruginosa MexXY protein, Pseudomonas aeruginosa OprJ protein, Pseudomonas aeruginosa OprM protein, Pseudomonas aeruginosa AmpC beta-lactamases beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Okamoto Kiyomi
Department of Microbiology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan.
Gotoh Naomasa
Nishino Takeshi
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.46.8.2696-2699.2002
Published
2002-08-00
Pages
2696-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC127373
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.46.8.2696-2699.2002 PMC Full Text View journal details
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