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PMID: 12196579 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neocortical long-term potentiation and long-term depression: site of expression investigated by infrared-guided laser stimulation.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 22 ·No. 17 ·2002-09-01 ·Pages 7558-68

Eder M, Zieglgänsberger W, Dodt HU

Abstract

The synaptic site of expression of long-term potentiation (LTP) and long-term depression (LTD) is still a matter of debate. To address the question of presynaptic versus postsynaptic expression of neocortical LTP and LTD in a direct approach, we measured the glutamate sensitivity of apical dendrites of layer 5 pyramidal neurons during LTP and LTD. We used infrared-guided laser stimulation to release glutamate from its "caged" form with high spatial and temporal resolution. Responses to photolytically released glutamate and synaptically evoked EPSPs were recorded with patch-clamp pipettes from the neuronal somata. LTP and LTD could be induced by electrical stimulation at the same synapses in succession. The NMDA receptor-dependent LTD was accompanied by a decrease in the dendritic glutamate sensitivity, suggesting a postsynaptic expression of neocortical LTD. In contrast, LTP was never accompanied by a change in the dendritic glutamate sensitivity. A possible explanation for this finding is a presynaptic expression of neocortical LTP. Another set of experiments corroborated these results: Photolytic application of glutamate with a frequency of 5 Hz caused a long-lasting Ca2+ and NMDA receptor-dependent decrease in the dendritic glutamate sensitivity. In contrast, LTP of dendritic glutamate sensitivity was never induced by photostimulation, despite several experimental modifications to prevent washout of the induction mechanism and to induce a stronger postsynaptic Ca2+ influx. In conclusion, our findings provide strong evidence for a postsynaptic expression of neocortical LTD and favor a primarily presynaptic locus of neocortical LTP.

MeSH Terms
Animals Calcium/metabolism Dendrites/drug effects,physiology,radiation effects Electric Stimulation Excitatory Amino Acid Antagonists/pharmacology Excitatory Postsynaptic Potentials/drug effects,radiation effects Glutamates/pharmacology,radiation effects In Vitro Techniques Infrared Rays Lasers Light Long-Term Potentiation/drug effects,physiology,radiation effects Male Neocortex/drug effects,physiology,radiation effects Neural Inhibition/drug effects,physiology,radiation effects Patch-Clamp Techniques Photic Stimulation/instrumentation,methods Photolysis Pyramidal Cells/drug effects,physiology,radiation effects Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors Sensitivity and Specificity Synapses/drug effects,physiology,radiation effects Ultraviolet Rays
Chemicals
(carboxy-2-nitrobenzyl)glutamic acid Excitatory Amino Acid Antagonists Glutamates Receptors, N-Methyl-D-Aspartate Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eder Matthias
Department of Clinical Neuropharmacology, Max-Planck-Institute of Psychiatry, 80804 Munich, Germany. [email protected]
Zieglgänsberger Walter
Dodt Hans-Ulrich
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Email
[email protected]
Published
2002-09-01
Pages
7558-68
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6758004
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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