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PMID: 12198156 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI.

The EMBO journal ·Vol. 21 ·No. 17 ·2002-09-02 ·Pages 4539-49

Rivero F, Illenberger D, Somesh BP, Dislich H, Adam N, Meyer AK

Abstract

Rho GDP-dissociation inhibitors (RhoGDIs) modulate the cycling of Rho GTPases between active GTP-bound and inactive GDP-bound states. We identified two RhoGDI homologues in DICTYOSTELIUM: GDI1 shares 51-58% similarity to RhoGDIs from diverse species. GDI2 is more divergent (40-44% similarity) and lacks the N-terminal regulatory arm characteristic for RhoGDI proteins. Both are cytosolic proteins and do not relocalize upon reorganization of the actin cytoskeleton. Using a two-hybrid approach, we identified Rac1a/1b/1c, RacB, RacC and RacE as interacting partners for GDI1. Cells lacking GDI1 are multinucleate, grow slowly and display a moderate pinocytosis defect, but rates of phagocytosis are unaffected. Mutant cells present prominent actin-rich protrusions, and large vacuoles that are continuous with the contractile vacuole system. The actin polymerization response upon stimulation with cAMP was reduced, but the motile behavior toward the chemoattractant was unaffected. Our results indicate that GDI1 plays a central role in the regulation of signal transduction cascades mediated by Rho GTPases.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Biopolymers Cell Division/genetics Chemotaxis/physiology Cytochalasins/pharmacology Cytoskeleton/metabolism,ultrastructure Dictyostelium/cytology,genetics,metabolism,ultrastructure Guanine Nucleotide Dissociation Inhibitors/deficiency,genetics,physiology Molecular Sequence Data Protozoan Proteins/genetics,physiology Sequence Alignment Sequence Homology, Amino Acid Signal Transduction Species Specificity Two-Hybrid System Techniques Vacuolar Proton-Translocating ATPases/metabolism Vacuoles/physiology rac GTP-Binding Proteins rho GTP-Binding Proteins/metabolism rho-Specific Guanine Nucleotide Dissociation Inhibitors
Chemicals
Actins Biopolymers Cytochalasins Guanine Nucleotide Dissociation Inhibitors Protozoan Proteins rho-Specific Guanine Nucleotide Dissociation Inhibitors cytochalasin A Vacuolar Proton-Translocating ATPases rac GTP-Binding Proteins rho GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rivero Francisco
Institut für Biochemie I, Medizinische Fakultät, University of Cologne, Joseph-Stelzmann-Strasse 52, D-50931 Köln, Germany. [email protected]
Illenberger Daria
Somesh Baggavalli P
Dislich Heidrun
Adam Nicola
Meyer Ann-Kathrin
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf449
Email
[email protected]
Published
2002-09-02
Pages
4539-49
Language
English
Region
England
NLM ID
8208664
PMCID
PMC126189
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf449 PMC Full Text View journal details
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