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PMID: 12207689 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S. Review

The unfolding story of the Escherichia coli Hsp70 DnaK: is DnaK a holdase or an unfoldase?

Molecular microbiology ·Vol. 45 ·No. 5 ·2002-09-00 ·Pages 1197-206

Slepenkov SV, Witt SN

Abstract

We discuss recent experiments that have illuminated individual steps in the reaction cycle of the Escherichia coli Hsp70 molecular chaperone DnaK. Using this new information, we compare two distinctly different global mechanisms of action--holding versus unfolding--and argue that the available evidence suggests that DnaK is an unfoldase.

MeSH Terms

Adenosine Triphosphate/metabolism Computer Simulation Escherichia coli Proteins/chemistry,genetics,metabolism HSP70 Heat-Shock Proteins/chemistry,metabolism Models, Biological Models, Molecular Molecular Chaperones/chemistry,metabolism Protein Biosynthesis Protein Folding Protein Processing, Post-Translational Protein Structure, Tertiary

Chemicals

Escherichia coli Proteins HSP70 Heat-Shock Proteins Molecular Chaperones Adenosine Triphosphate dnaK protein, E coli

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Slepenkov Sergey V

Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130-3932, USA.

Witt Stephan N

Article Info

Journal

Molecular microbiology

Abbr.

Mol Microbiol

ISSN

0950-382X

DOI

10.1046/j.1365-2958.2002.03093.x

Published

2002-09-00

Pages

1197-206

Language

English

Region

England

NLM ID

8712028

Subset

Grants

NIGMS NIH HHS · GM 51521 · United States

External Links

PubMed source DOI: 10.1046/j.1365-2958.2002.03093.x View journal details

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The unfolding story of the Escherichia coli Hsp70 DnaK: is DnaK a holdase or an unfoldase?

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