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PMID: 12215650 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Little evidence for developmental plasticity of adult hematopoietic stem cells.

Science (New York, N.Y.) ·Vol. 297 ·No. 5590 ·2002-09-27 ·Pages 2256-9

Wagers AJ, Sherwood RI, Christensen JL, Weissman IL

Abstract

To rigorously test the in vivo cell fate specificity of bone marrow (BM) hematopoietic stem cells (HSCs), we generated chimeric animals by transplantation of a single green fluorescent protein (GFP)-marked HSC into lethally irradiated nontransgenic recipients. Single HSCs robustly reconstituted peripheral blood leukocytes in these animals, but did not contribute appreciably to nonhematopoietic tissues, including brain, kidney, gut, liver, and muscle. Similarly, in GFP+:GFP- parabiotic mice, we found substantial chimerism of hematopoietic but not nonhematopoietic cells. These data indicate that "transdifferentiation" of circulating HSCs and/or their progeny is an extremely rare event, if it occurs at all.

MeSH Terms

Animals Brain/cytology Cell Differentiation Cell Lineage Chimera Fluorescent Antibody Technique Green Fluorescent Proteins Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells/cytology,physiology Hepatocytes/cytology Intestinal Mucosa/cytology,radiation effects Intestine, Large/cytology Intestine, Small/cytology Kidney/cytology Leukocyte Common Antigens/analysis Luminescent Proteins/analysis Lung/cytology Mice Mice, Inbred C57BL Mice, Transgenic Muscle, Skeletal/cytology Myocardium/cytology Neurons/cytology Parabiosis Regeneration Stem Cells/cytology,physiology

Chemicals

Luminescent Proteins Green Fluorescent Proteins Leukocyte Common Antigens

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wagers Amy J

Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA. [email protected]

Sherwood Richard I

Christensen Julie L

Weissman Irving L

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1074807

[email protected]

Published

2002-09-27

Epub

2002-00-05

Pages

2256-9

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NIAID NIH HHS · 5T32AI07290-16 · United States

NCI NIH HHS · CA-86065 · United States

Corrections

CommentIn

PMID: 12392027

CommentIn

PMID: 12610282

External Links

PubMed source DOI: 10.1126/science.1074807 View journal details

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Little evidence for developmental plasticity of adult hematopoietic stem cells.

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