How to distinguish between the vacuum cleaner and flippase mechanisms of the lmrA multi-drug transporter in Lactococcus lactis.

Molecular biology reports ·Vol. 29 ·No. 1-2 ·2002-00-00 ·Pages 107-12

Hofmeyr JH, Rohwer JM, Snoep JL, Westerhoff HV, Konings WN

Abstract

A numerical model of the LmrA multi-drug transport system of Lactococcus lactis is used to explore the possibility of distinguishing experimentally between two putative transport mechanisms, i.e., the vacuum-cleaner and the flippase mechanisms. This comparative model also serves as an example of numerical simulation with the scripting language Python and its scientific add-on Scipy.

MeSH Terms

Bacterial Proteins/metabolism Computer Simulation Drug Resistance, Multiple Lactococcus lactis/metabolism Mathematics Models, Biological Multidrug Resistance-Associated Proteins/metabolism Software

Chemicals

Bacterial Proteins LmrA protein, Lactococcus lactis Multidrug Resistance-Associated Proteins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Hofmeyr J H S

Dept of Biochemistry, University of Stellenbosch, South Africa. [email protected]

Rohwer J M

Snoep J L

Westerhoff H V

Konings W N

References (4)

4 references, click to expand

Modelling cellular processes with Python and Scipy.
Mol Biol Rep. 2002;29(1-2):249-54 PMID: 12241066
Proton motive force-driven and ATP-dependent drug extrusion systems in multidrug-resistant Lactococcus lactis.
J Bacteriol. 1994 Nov;176(22):6957-64 PMID: 7961458
Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1.
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10668-72 PMID: 8855237
Multidrug resistance in Lactococcus lactis: evidence for ATP-dependent drug extrusion from the inner leaflet of the cytoplasmic membrane.
EMBO J. 1996 Aug 15;15(16):4239-45 PMID: 8861952

Article Info

Journal

Molecular biology reports

Abbr.

Mol Biol Rep

ISSN

0301-4851

DOI

10.1023/a:1020344922249

[email protected]

Published

2002-00-00

Pages

107-12

Language

English

Region

Netherlands

NLM ID

0403234

Subset

External Links

PubMed source DOI: 10.1023/a:1020344922249 View journal details

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How to distinguish between the vacuum cleaner and flippase mechanisms of the lmrA multi-drug transporter in Lactococcus lactis.

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