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PMID: 123001 Published · ppublish English Comparative Study Journal Article

The surface morphology of human B lymphocytes as revealed by immunoelectron microscopy.

The Journal of experimental medicine ·Vol. 141 ·No. 2 ·1975-02-01 ·Pages 392-410

Lejonc RJ, Gourdin MF, Mannoni P, Dreyfus B, Reyes F

Abstract

Surface immunoglobulins (sIg) were detected on human lymphocytes by immunoelectron microscopy with peroxidase-conjugated antibodies. Blood, marrow, and thymus cells from normal individuals and patients with lymphoproliferative disorders were examined. Samples were fixed before exposure to specific reagents. Normal lymphocyts with detectable sIg, i.e. B lymphocytes, were characterized by a villous surface; nonlabeled blood lymphocytes and thymocytes were smooth cells. Intermediate cells were also found which in sections appeared moderately villous and labeled, thus identified as B lymphocytes. Further evidence for a relationship between villous surface and sIg was given by the finding of a few lymphocytes with polar concentration of labeled microvilli. In chronic lymphocytic leukemia patients, most cells exhibited a villous surface with parallel variations of the number of microvilli and of anti-immunoglobulin-binding capacity. However, some labeled smooth blastic cells were also observed. On the other hand, abnormal lymphocytes from Sézary's syndrome which could exhibit segments of villous membrane had no detectable sIg. This study confirms that in most cases human B lymphocytes have a characteristic surface appearance and that the detection of sIg in normal lymphocytes correlates with the presence of microvilli.

MeSH Terms
B-Lymphocytes/ultrastructure Binding Sites, Antibody Bone Marrow/ultrastructure Bone Marrow Cells Cell Membrane/ultrastructure Dermatitis, Exfoliative/blood Humans Immunoglobulin G/analysis Immunoglobulin M/analysis Immunologic Techniques Keratoderma, Palmoplantar/blood Leukemia, Lymphoid/blood Lymphatic Diseases/blood Microscopy, Electron Peroxidases Syndrome Thymus Gland/cytology
Chemicals
Immunoglobulin G Immunoglobulin M Peroxidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lejonc R J
Gourdin M F
Mannoni P
Dreyfus B
Reyes F
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.141.2.392
Published
1975-02-01
Pages
392-410
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190536
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.141.2.392 PMC Full Text View journal details
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