Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction.

Adaptor Proteins, Signal Transducing Antigens, Differentiation/metabolism Antigens, Surface/metabolism Blotting, Western Brefeldin A/pharmacology Cell Line Cell Separation Dose-Response Relationship, Drug Drosophila Proteins Escherichia coli/metabolism Flow Cytometry Fluorescent Dyes/pharmacology Genes, Reporter Golgi Apparatus/metabolism Green Fluorescent Proteins Humans Lipopolysaccharide Receptors/metabolism Lipopolysaccharides/metabolism Luciferases/metabolism Luminescent Proteins/metabolism Lymphocyte Antigen 96 Membrane Glycoproteins/metabolism Microscopy, Confocal Microscopy, Fluorescence Myeloid Differentiation Factor 88 Plasmids/metabolism Precipitin Tests Protein Binding Protein Structure, Tertiary Protein Transport Receptors, Cell Surface/metabolism Receptors, Immunologic/metabolism Recombinant Fusion Proteins/metabolism Signal Transduction Time Factors Toll-Like Receptor 4 Toll-Like Receptors Transfection

Adaptor Proteins, Signal Transducing Antigens, Differentiation Antigens, Surface Drosophila Proteins Fluorescent Dyes LY96 protein, human Lipopolysaccharide Receptors Lipopolysaccharides Luminescent Proteins Lymphocyte Antigen 96 MYD88 protein, human Membrane Glycoproteins Myeloid Differentiation Factor 88 Receptors, Cell Surface Receptors, Immunologic Recombinant Fusion Proteins TLR4 protein, human Toll-Like Receptor 4 Toll-Like Receptors Green Fluorescent Proteins Brefeldin A Luciferases