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PMID: 12351682 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Trypanosoma cruzi expresses a plant-like ascorbate-dependent hemoperoxidase localized to the endoplasmic reticulum.

Wilkinson SR, Obado SO, Mauricio IL, Kelly JM

Abstract

In most aerobic organisms hemoperoxidases play a major role in H(2)O(2)-detoxification, but trypanosomatids have been reported to lack this activity. Here we describe the properties of an ascorbate-dependent hemoperoxidase (TcAPX) from the American trypanosome Trypanosoma cruzi. The activity of this plant-like enzyme can be linked to the reduction of the parasite-specific thiol trypanothione by ascorbate in a process that involves nonenzymatic interaction. The role of heme in peroxidase activity was demonstrated by spectral and inhibition studies. Ascorbate could saturate TcAPX activity indicating that the enzyme obeys Michaelis-Menten kinetics. Parasites that overexpressed TcAPX activity were found to have increased resistance to exogenous H(2)O(2). To determine subcellular location an epitope-tagged form of TcAPX was expressed in T. cruzi, which was observed to colocalize with endoplasmic reticulum resident chaperone protein BiP. These findings identify an arm of the oxidative defense system of this medically important parasite. The absence of this redox pathway in the human host may be therapeutically exploitable.

MeSH Terms
Amino Acid Sequence Animals Ascorbate Peroxidases Ascorbic Acid/metabolism Base Sequence DNA, Protozoan/genetics Endoplasmic Reticulum/enzymology Genes, Protozoan Humans Hydrogen Peroxide/metabolism,pharmacology Molecular Sequence Data Oxidation-Reduction Peroxidases/chemistry,genetics,metabolism Plants/enzymology Sequence Homology, Amino Acid Trypanosoma cruzi/drug effects,enzymology,genetics
Chemicals
DNA, Protozoan Hydrogen Peroxide Peroxidases Ascorbate Peroxidases Ascorbic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wilkinson Shane R
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK. [email protected]
Obado Samson O
Mauricio Isabel L
Kelly John M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
2002-10-15
Epub
2002-00-26
Pages
13453-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC129694
Subset
IM
Databases
GENBANK
AJ457987
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