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PMID: 12368509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells.

The Plant cell ·Vol. 14 ·No. 10 ·2002-10-00 ·Pages 2627-41

Lecourieux D, Mazars C, Pauly N, Ranjeva R, Pugin A

Abstract

Cell suspensions obtained from Nicotiana plumbaginifolia plants stably expressing the apoaequorin gene were used to analyze changes in cytosolic free calcium concentrations ([Ca(2+)](cyt)) in response to elicitors of plant defenses, particularly cryptogein and oligogalacturonides. The calcium signatures differ in lag time, peak time, intensity, and duration. The intensities of both signatures depend on elicitor concentration and extracellular calcium concentration. Cryptogein signature is characterized by a long-sustained [Ca(2+)](cyt) increase that should be responsible for sustained mitogen-activated protein kinase activation, microtubule depolymerization, defense gene activation, and cell death. The [Ca(2+)](cyt) increase in elicitor-treated cells first results from a calcium influx, which in turns leads to calcium release from internal stores and additional Ca(2+) influx. H(2)O(2) resulting from the calcium-dependent activation of the NADPH oxidase also participates in [Ca(2+)](cyt) increase and may activate calcium channels from the plasma membrane. Competition assays with different elicitins demonstrate that [Ca(2+)](cyt) increase is mediated by cryptogein-receptor interaction.

MeSH Terms
Aequorin/genetics,metabolism Algal Proteins/pharmacology Apoproteins/genetics,metabolism Apoptosis/drug effects Calcium/metabolism Calcium-Binding Proteins/drug effects,genetics,metabolism Cytosol/metabolism Fungal Proteins/pharmacology Gene Expression Regulation/drug effects Glucans Hydrogen Peroxide/metabolism Lipopolysaccharides/pharmacology Microtubules/drug effects Mitogen-Activated Protein Kinases/metabolism NADPH Oxidases/metabolism Neomycin/pharmacology Oligosaccharides/chemistry,pharmacology Phosphorylation Plants, Genetically Modified Polysaccharides/pharmacology Proteins/pharmacology Recombinant Proteins/genetics,metabolism Ribosome Inactivating Proteins, Type 2 Tobacco/cytology,drug effects,metabolism Transcriptional Activation
Chemicals
Algal Proteins Apoproteins Calcium-Binding Proteins Fungal Proteins Glucans Lipopolysaccharides Oligosaccharides Polysaccharides Proteins Recombinant Proteins Ribosome Inactivating Proteins, Type 2 apoaequorin cryptogein protein, Phytophthora cryptogea oligogalacturonic acid cinnamomin, Phytophthora cinnamomi Alpha-elicitin capsicein protein, Phytophthora capsici Aequorin laminaran Hydrogen Peroxide NADPH Oxidases Mitogen-Activated Protein Kinases Neomycin Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lecourieux David
Unité Mixte de Recherche Institut National de la Recherche Agronomique-Université de Bourgogne, Biochimie, Biologie Cellulaire et Ecologie des Interactions Plantes-Microorganismes, 17 rue de Sully, BP 86510, 21065 Dijon cedex, France.
Mazars Christian
Pauly Nicolas
Ranjeva Raoul
Pugin Alain
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.005579
Published
2002-10-00
Pages
2627-41
Language
English
Region
England
NLM ID
9208688
PMCID
PMC151240
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.005579 PMC Full Text View journal details
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