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PMID: 12374734 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functions of LIM proteins in cell polarity and chemotactic motility.

The EMBO journal ·Vol. 21 ·No. 20 ·2002-10-15 ·Pages 5331-42

Khurana B, Khurana T, Khaire N, Noegel AA

Abstract

LimC and LimD are two novel LIM proteins of Dictyostelium, which are comprised of double and single LIM domains, respectively. Green fluorescent protein-fused LimC and LimD proteins preferentially accumulate at areas of the cell cortex where they co-localize with actin and associate transiently with cytoskeleton-dependent dynamic structures like phagosomes, macropinosomes and pseudopods. Furthermore, both LimC and LimD interact directly with F-actin in vitro. Mutant cells that lack either LimC or LimD, or both, exhibit normal growth. They are, however, significantly impaired in growth under stress conditions and are highly sensitive to osmotic shock, suggesting that LimC and LimD contribute towards the maintenance of cortical strength. Moreover, we noted an altered morphology and F-actin distribution in LimD(-) and LimC(-)/D(-) mutants, and changes in chemotactic motility associated with an increased pseudopod formation. Our results reveal both unique and overlapping roles for LimC and LimD, and suggest that both act directly on the actin cytoskeleton and provide rigidity to the cortex.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Cell Division Cell Polarity/physiology Chemotaxis/physiology Cytoskeleton/metabolism Dictyostelium/cytology,genetics,physiology Endocytosis/physiology Genes, Protozoan Green Fluorescent Proteins Luminescent Proteins/chemistry,genetics,metabolism Molecular Sequence Data Mutation Protozoan Proteins/chemistry,genetics,physiology Recombinant Fusion Proteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid Subcellular Fractions/metabolism
Chemicals
Actins Luminescent Proteins Protozoan Proteins Recombinant Fusion Proteins Green Fluorescent Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Khurana Bharat
Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, D-50931 Cologne, Germany.
Khurana Taruna
Khaire Nandkumar
Noegel Angelika A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf550
Published
2002-10-15
Pages
5331-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC129082
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf550 PMC Full Text View journal details
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