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PMID: 12376619 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protection of the intestinal mucosa by intraepithelial gamma delta T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 22 ·2002-10-29 ·Pages 14338-43

Chen Y, Chou K, Fuchs E, Havran WL, Boismenu R

Abstract

gammadelta intraepithelial T lymphocytes (IEL) represent a major T cell population within the intestine of unclear functional relevance. The role of intestinal gammadelta IEL was evaluated in the dextran sodium sulfate (DSS) induced mouse colitis model system. Large numbers of gammadelta T cells, but not alphabeta T cells, were localized at sites of DSS-induced epithelial cell damage. gammadelta IEL in DSS treated mice expressed keratinocyte growth factor (KGF), a potent intestinal epithelial cell mitogen. gammadelta cell-deficient mice (TCRdelta(-/-)) and KGF-deficient mice (KGF(-/-)), but not alphabeta cell-deficient mice (TCRalpha(-/-)), were more prone than wild-type mice to DSS-induced mucosal injury and demonstrated delayed tissue repair after termination of DSS treatment. Termination of DSS treatment resulted in vigorous epithelial cell proliferation in wild-type mice but not in TCRdelta(-/-) mice or KGF(-/-) mice. These results suggest that gammadelta IEL help preserve the integrity of damaged epithelial surfaces by providing the localized delivery of an epithelial cell growth factor.

MeSH Terms
Animals Cell Division Colitis/chemically induced,immunology,pathology Dextran Sulfate/adverse effects Disease Models, Animal Epithelial Cells/cytology Fibroblast Growth Factor 7 Fibroblast Growth Factors/genetics,immunology Genetic Predisposition to Disease/genetics Intestinal Mucosa/cytology,immunology Mice Mice, Inbred C57BL Mice, Knockout Receptors, Antigen, T-Cell, gamma-delta/genetics,immunology T-Lymphocytes/immunology Time Factors
Chemicals
Fgf7 protein, mouse Receptors, Antigen, T-Cell, gamma-delta Fibroblast Growth Factor 7 Fibroblast Growth Factors Dextran Sulfate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chen Yaping
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Chou Kevin
Fuchs Elaine
Havran Wendy L
Boismenu Richard
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.212290499
Published
2002-10-29
Epub
2002-00-10
Pages
14338-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC137885
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.212290499 PMC Full Text View journal details
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