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PMID: 12376630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Phase-specific circadian clock regulatory elements in Arabidopsis.

Plant physiology ·Vol. 130 ·No. 2 ·2002-10-00 ·Pages 627-38

Michael TP, McClung CR

Abstract

We have defined a minimal Arabidopsis CATALASE 3 (CAT3) promoter sufficient to drive evening-specific circadian transcription of a LUCIFERASE reporter gene. Deletion analysis and site-directed mutagenesis reveal a circadian response element, the evening element (EE: AAAATATCT), that is necessary for evening-specific transcription. The EE differs only by a single base pair from the CIRCADIAN CLOCK ASSOCIATED 1-binding site (CBS: AAAAAATCT), which is important for morning-specific transcription. We tested the hypothesis that the EE and the CBS specify circadian phase by site-directed mutagenesis to convert the CAT3 EE into a CBS. Changing the CAT3 EE to a CBS changes the phase of peak transcription from the evening to the morning in continuous dark and in light-dark cycles, consistent with the specification of phase by the single base pair that distinguishes these elements. However, rhythmicity of the CBS-containing CAT3 promoter is dramatically compromised in continuous light. Thus, we conclude that additional information normally provided in the context of a morning-specific promoter is necessary for full circadian activity of the CBS.

MeSH Terms
Arabidopsis/genetics,growth & development,radiation effects Arabidopsis Proteins/genetics,metabolism Binding Sites/genetics Catalase/genetics,metabolism Circadian Rhythm/genetics,physiology Darkness Gene Deletion Gene Expression Regulation, Plant Light Luciferases/genetics,metabolism Mutagenesis, Site-Directed Plants, Genetically Modified Promoter Regions, Genetic/genetics Regulatory Sequences, Nucleic Acid/genetics Response Elements/genetics Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Arabidopsis Proteins CCA1 protein, Arabidopsis Transcription Factors Catalase Luciferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Michael Todd P
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
McClung C Robertson
References (61)
61 references, click to expand
  1. A role for LKP2 in the circadian clock of Arabidopsis.
    Plant Cell. 2001 Dec;13(12):2659-70 PMID: 11752379
  2. Squaring up the E-box.
    J Biol Rhythms. 2000 Dec;15(6):483-90 PMID: 11106065
  3. The Atger3 promoter confers circadian clock-regulated transcription with peak expression at the beginning of the night.
    Plant Mol Biol. 1999 Jul;40(5):873-82 PMID: 10487221
  4. Circadian programs of transcriptional activation, signaling, and protein turnover revealed by microarray analysis of mammalian cells.
    Curr Biol. 2002 Apr 2;12(7):551-7 PMID: 11937023
  5. Orchestrated transcription of key pathways in Arabidopsis by the circadian clock.
    Science. 2000 Dec 15;290(5499):2110-3 PMID: 11118138
  6. Circadian clock mutants in Arabidopsis identified by luciferase imaging.
    Science. 1995 Feb 24;267(5201):1161-3 PMID: 7855595
  7. A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock.
    Cell. 1999 Jan 8;96(1):57-68 PMID: 9989497
  8. Regulation of c-fos expression in transgenic mice requires multiple interdependent transcription control elements.
    Neuron. 1995 Feb;14(2):241-52 PMID: 7857636
  9. The period E-box is sufficient to drive circadian oscillation of transcription in vivo.
    J Biol Rhythms. 2000 Dec;15(6):462-71 PMID: 11106063
  10. The 69 bp circadian regulatory sequence (CRS) mediates per-like developmental, spatial, and circadian expression and behavioral rescue in Drosophila.
    J Neurosci. 1999 Feb 1;19(3):987-94 PMID: 9920662
  11. Circadian cycling of the mouse liver transcriptome, as revealed by cDNA microarray, is driven by the suprachiasmatic nucleus.
    Curr Biol. 2002 Apr 2;12(7):540-50 PMID: 11937022
  12. The Drosophila dCREB2 gene affects the circadian clock.
    Neuron. 1999 Apr;22(4):777-87 PMID: 10230797
  13. Protein kinase CK2 interacts with and phosphorylates the Arabidopsis circadian clock-associated 1 protein.
    Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):11020-5 PMID: 9724822
  14. Circadian clock-regulated expression of an RNA-binding protein in Arabidopsis: characterisation of a minimal promoter element.
    Mol Gen Genet. 1999 Jun;261(4-5):811-9 PMID: 10394919
  15. Multiple DNA-Protein Complexes at a Circadian-Regulated Promoter Element.
    Plant Cell. 1995 Dec;7(12):2039-2051 PMID: 12242368
  16. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
    Plant J. 1998 Dec;16(6):735-43 PMID: 10069079
  17. Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock.
    Plant Cell Physiol. 2000 Sep;41(9):1002-12 PMID: 11100772
  18. Role of the CLOCK protein in the mammalian circadian mechanism.
    Science. 1998 Jun 5;280(5369):1564-9 PMID: 9616112
  19. The late elongated hypocotyl mutation of Arabidopsis disrupts circadian rhythms and the photoperiodic control of flowering.
    Cell. 1998 Jun 26;93(7):1219-29 PMID: 9657154
  20. A light- and temperature-entrained circadian clock controls expression of transcripts encoding nuclear proteins with homology to RNA-binding proteins in meristematic tissue.
    Plant J. 1994 Jun;5(6):799-813 PMID: 8054987
  21. The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins.
    Trends Biochem Sci. 1995 Dec;20(12):506-10 PMID: 8571452
  22. DNA binding activity of the Arabidopsis G-box binding factor GBF1 is stimulated by phosphorylation by casein kinase II from broccoli.
    Plant Cell. 1992 Jan;4(1):87-98 PMID: 1525562
  23. Circadian regulation of cAMP response element-mediated gene expression in the suprachiasmatic nuclei.
    J Biol Chem. 1999 Jun 18;274(25):17748-56 PMID: 10364217
  24. Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim.
    Science. 1998 Jun 5;280(5369):1599-603 PMID: 9616122
  25. Quantitative analysis of Drosophila period gene transcription in living animals.
    J Biol Rhythms. 1997 Jun;12(3):204-17 PMID: 9181432
  26. Effects of synergistic signaling by phytochrome A and cryptochrome1 on circadian clock-regulated catalase expression.
    Plant Cell. 1997 Jun;9(6):947-55 PMID: 9212468
  27. Combinatorial interplay of promoter elements constitutes the minimal determinants for light and developmental control of gene expression in Arabidopsis.
    EMBO J. 1996 Jul 15;15(14):3732-43 PMID: 8670877
  28. Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog.
    Science. 2000 Aug 4;289(5480):768-71 PMID: 10926537
  29. Reconstitution of Arabidopsis casein kinase II from recombinant subunits and phosphorylation of transcription factor GBF1.
    Plant Cell. 1995 Jan;7(1):105-15 PMID: 7696877
  30. A novel circadian phenotype based on firefly luciferase expression in transgenic plants.
    Plant Cell. 1992 Sep;4(9):1075-87 PMID: 1392609
  31. Constitutive expression of the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene disrupts circadian rhythms and suppresses its own expression.
    Cell. 1998 Jun 26;93(7):1207-17 PMID: 9657153
  32. Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis.
    Plant Cell. 2001 Jan;13(1):113-23 PMID: 11158533
  33. The short-period mutant, toc1-1, alters circadian clock regulation of multiple outputs throughout development in Arabidopsis thaliana.
    Development. 1998 Feb;125(3):485-94 PMID: 9425143
  34. Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana.
    Plant Cell Physiol. 2000 Jun;41(6):791-803 PMID: 10945350
  35. The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: II. Characterization with CCA1-overexpressing plants.
    Plant Cell Physiol. 2002 Jan;43(1):118-22 PMID: 11828029
  36. A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.
    Plant Cell. 1997 Apr;9(4):491-507 PMID: 9144958
  37. ELF3 modulates resetting of the circadian clock in Arabidopsis.
    Plant Cell. 2001 Jun;13(6):1305-15 PMID: 11402162
  38. Circadian clock-regulated expression of phytochrome and cryptochrome genes in Arabidopsis.
    Plant Physiol. 2001 Dec;127(4):1607-16 PMID: 11743105
  39. Specific sequences outside the E-box are required for proper per expression and behavioral rescue.
    J Biol Rhythms. 2000 Dec;15(6):472-82 PMID: 11106064
  40. Interconnected feedback loops in the Neurospora circadian system.
    Science. 2000 Jul 7;289(5476):107-10 PMID: 10884222
  41. Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock.
    Science. 2001 Aug 3;293(5531):880-3 PMID: 11486091
  42. Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter.
    Mol Cell Biol. 2001 Feb;21(4):1207-17 PMID: 11158307
  43. DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1.
    EMBO J. 1992 Apr;11(4):1275-89 PMID: 1563344
  44. Conditional circadian regulation of PHYTOCHROME A gene expression.
    Plant Physiol. 2001 Dec;127(4):1808-18 PMID: 11743124
  45. A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster.
    Mol Cell Biol. 1997 Jul;17(7):3687-93 PMID: 9199302
  46. The protein kinase CK2 is involved in regulation of circadian rhythms in Arabidopsis.
    Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12362-6 PMID: 10535927
  47. Interacting molecular loops in the mammalian circadian clock.
    Science. 2000 May 12;288(5468):1013-9 PMID: 10807566
  48. Positive and negative regulation of the rat vasopressin gene promoter.
    Endocrinology. 1997 Dec;138(12):5266-74 PMID: 9389510
  49. A clock- and light-regulated gene that links the circadian oscillator to LHCB gene expression.
    Plant Cell. 2001 Jun;13(6):1411-25 PMID: 11402169
  50. Combinatorial interaction of light-responsive elements plays a critical role in determining the response characteristics of light-regulated promoters in Arabidopsis.
    Plant J. 1998 Jul;15(1):69-77 PMID: 9744096
  51. Circadian regulation of diverse gene products revealed by mRNA expression profiling of synchronized fibroblasts.
    J Biol Chem. 2001 Dec 14;276(50):46751-8 PMID: 11598123
  52. Integration of circadian and phototransduction pathways in the network controlling CAB gene transcription in Arabidopsis.
    Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15491-6 PMID: 8986839
  53. Circadian orchestration of gene expression in cyanobacteria.
    Genes Dev. 1995 Jun 15;9(12):1469-78 PMID: 7601351
  54. Interlocked feedback loops within the Drosophila circadian oscillator.
    Science. 1999 Oct 22;286(5440):766-8 PMID: 10531060
  55. The circadian clock gates expression of two Arabidopsis catalase genes to distinct and opposite circadian phases.
    Mol Gen Genet. 1996 May 23;251(2):196-203 PMID: 8668130
  56. Circadian regulation of gene expression systems in the Drosophila head.
    Neuron. 2001 Nov 20;32(4):657-71 PMID: 11719206
  57. Microarray analysis and organization of circadian gene expression in Drosophila.
    Cell. 2001 Nov 30;107(5):567-78 PMID: 11733057
  58. Identification of tomato Lhc promoter regions necessary for circadian expression.
    Plant Mol Biol. 1998 Nov 1;38(4):655-62 PMID: 9747810
  59. The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants.
    Plant Cell Physiol. 2002 Jan;43(1):58-69 PMID: 11828023
  60. Molecular bases for circadian clocks.
    Cell. 1999 Jan 22;96(2):271-90 PMID: 9988221
  61. Identification of an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase/oxygenase activase (RCA) minimal promoter regulated by light and the circadian clock.
    Plant Physiol. 1996 Sep;112(1):43-51 PMID: 8819320
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.004929
Published
2002-10-00
Pages
627-38
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC166592
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.004929 PMC Full Text View journal details
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