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PMID: 12426375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans.

The EMBO journal ·Vol. 21 ·No. 22 ·2002-11-15 ·Pages 6025-35

Tonkin LA, Saccomanno L, Morse DP, Brodigan T, Krause M, Bass BL

Abstract

Here we take advantage of the well-characterized and simple nervous system of Caenorhabditis elegans to further our understanding of the functions of RNA editing. We describe the two C.elegans ADAR genes, adr-1 and adr-2, and characterize strains containing homozygous deletions in each, or both, of these genes. We find that adr-1 is expressed in most, if not all, cells of the C.elegans nervous system and also in the developing vulva. Using chemotaxis assays, we show that both ADARs are important for normal behavior. Biochemical, molecular and phenotypic analyses indicate that ADR-1 and ADR-2 have distinct roles in C.elegans, but sometimes act together.

MeSH Terms
Adenosine Deaminase/genetics,physiology Animals Behavior, Animal Caenorhabditis elegans/genetics,physiology Caenorhabditis elegans Proteins/genetics,physiology Catalysis Catalytic Domain Chemotaxis/genetics Diptera/metabolism Female Male Mammals/metabolism Organ Specificity Phenotype Pheromones/physiology RNA Editing RNA, Helminth/genetics,metabolism Sequence Deletion Species Specificity Structure-Activity Relationship Vulva/growth & development
Chemicals
Caenorhabditis elegans Proteins Pheromones RNA, Helminth Adenosine Deaminase Adr-1 protein, C elegans Adr-2 protein, C elegans
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tonkin Leath A
Department of Biochemistry and Howard Hughes Medical Institute, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA.
Saccomanno Lisa
Morse Daniel P
Brodigan Thomas
Krause Michael
Bass Brenda L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf607
Published
2002-11-15
Pages
6025-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC137199
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044073 · United States
NIGMS NIH HHS · R01 GM044073-11 · United States
NIGMS NIH HHS · GM 44073 · United States
NCI NIH HHS · CA 42014 · United States
External Links
PubMed source DOI: 10.1093/emboj/cdf607 PMC Full Text View journal details
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