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PMID: 12426401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

An Arabidopsis histidine kinase is essential for megagametogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 24 ·2002-11-26 ·Pages 15800-5

Pischke MS, Jones LG, Otsuga D, Fernandez DE, Drews GN, Sussman MR

Abstract

Cytokinin-Independent 1 (CKI1) belongs to a group of putative plant histidine kinases whose members do not appear to act as ethylene receptors. The deduced protein structure, combined with the observation that Arabidopsis callus cultures overexpressing CKI1 exhibit a "cytokinin-independent" cell division and greening phenotype, led to the hypothesis that CKI1 is involved in cytokinin signaling, perhaps acting as a cytokinin receptor. To test the function of CKI1, we used a reverse-genetic approach to identify plants carrying T-DNA insertions in CKI1. Two independent alleles were identified, which produce the same developmental phenotype. Analyses of populations segregating for the cki1-5 or cki1-6 T-DNA insertion alleles failed to reveal any homozygous cki1 plants, indicating that the homozygous mutant condition was lethal. Based on segregation distortion, transmission studies, a microscopy-based examination of developing female gametophytes, and mRNA expression data, we suggest that CKI1 function is required for megagametophyte development. Our work with CKI1 mutants indicates that signal transduction by means of a HisAsp phosphorelay system may play an important and previously unsuspected role in female gametophyte development in Arabidopsis.

MeSH Terms
Alleles Arabidopsis/enzymology,physiology Arabidopsis Proteins/genetics,physiology Cell Division DNA, Bacterial/genetics Gametogenesis/physiology Gene Expression Profiling Microscopy, Confocal Mutagenesis, Insertional Organ Culture Techniques Phenotype Phosphorylation Protein Kinases/genetics,physiology Protein Processing, Post-Translational RNA, Messenger/biosynthesis RNA, Plant/biosynthesis Signal Transduction
Chemicals
Arabidopsis Proteins DNA, Bacterial RNA, Messenger RNA, Plant T-DNA Protein Kinases CKI1 protein, Arabidopsis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pischke Melissa S
Biotechnology Center, University of Wisconsin, 425 Henry Mall, Madison 53706, USA.
Jones Linda G
Otsuga Denichiro
Fernandez Donna E
Drews Gary N
Sussman Michael R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.232580499
Published
2002-11-26
Epub
2002-00-08
Pages
15800-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC137796
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.232580499 PMC Full Text View journal details
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