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PMID: 124440 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mucopolysaccharides associated with nuclei of cultured mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2032-6

Bhavanandan VP, Davidson EA

Abstract

Mucopolysaccharides have been isolated, fractionated, and characterized from the nuclei of cultured B16 mouse melanoma cells grown in the presence of (3-H)-glucosamine and (35-S)sulfate. Digestion of the nuclei with DNase followed by Pronase gave a mixture of complex carbohydrates from which the mucopolysaccharides were isolated by precipitation with cetylpyridinium chloride. After fractionation by differential salt extraction and chromatography on controlled pore glass bead columns, the components were identified by chemical and enzymatic methods. The major polysaccharide components were a family of high-molecular-weight chondroitin sulfates with different degrees of sulfation; a minor component has been characterized as heparan sulfate.

MeSH Terms
Animals Bacteria/enzymology Cell Line Cell Nucleus/analysis Cetylpyridinium Chemical Precipitation Chondroitin/analysis,isolation & purification Chromatography Glycosaminoglycans/analysis,isolation & purification Hyaluronoglucosaminidase Lyases Male Melanoma/analysis,pathology Mice Pronase Sulfatases Testis/enzymology
Chemicals
Glycosaminoglycans Chondroitin Cetylpyridinium Sulfatases Hyaluronoglucosaminidase Pronase Lyases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhavanandan V P
Davidson E A
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2032
Published
1975-06-00
Pages
2032-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432686
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2032 PMC Full Text View journal details
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