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PMID: 12449370 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Gap repair transformation in fission yeast to exchange plasmid-selectable markers.

BioTechniques ·Vol. 33 ·No. 5 ·2002-11-00 ·Pages 978, 980, 982

Kelly DA, Hoffman CS

Abstract

暂无摘要

MeSH Terms
3-Isopropylmalate Dehydrogenase Alcohol Oxidoreductases/genetics Base Sequence Cloning, Molecular/methods DNA Repair DNA, Fungal/genetics Genetic Complementation Test Genetic Markers Genetic Vectors/genetics Molecular Sequence Data Polymerase Chain Reaction Promoter Regions, Genetic/genetics Saccharomyces cerevisiae Proteins/genetics Schizosaccharomyces/genetics Selection, Genetic Transformation, Genetic
Chemicals
DNA, Fungal Genetic Markers Saccharomyces cerevisiae Proteins Alcohol Oxidoreductases 3-Isopropylmalate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelly David A
Boston College, Chestnut Hill, MA 02467, USA.
Hoffman Charles S
References (9)
9 references, click to expand
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    Yeast. 1998 Jul;14 (10 ):943-51 PMID: 9717240
  3. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
    Gene. 1987;57(2-3):267-72 PMID: 3319781
  4. The S.pombe mei2 gene encoding a crucial molecule for commitment to meiosis is under the regulation of cAMP.
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  5. Yeast recombination: the association between double-strand gap repair and crossing-over.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4417-21 PMID: 6308623
  6. The best yeast?
    Trends Genet. 1999 Sep;15(9):340-4 PMID: 10461200
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    Nucleic Acids Res. 1998 Oct 15;26(20):4783-4 PMID: 9753750
  8. A rapid method for localized mutagenesis of yeast genes.
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  9. Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe.
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Article Info
Journal
BioTechniques
Abbr.
Biotechniques
ISSN
0736-6205
DOI
10.2144/02335bm02
Published
2002-11-00
Pages
978, 980, 982
Language
English
Region
England
NLM ID
8306785
PMCID
PMC4419568
Subset
IM
Grants
NIGMS NIH HHS · R01 GM046226-09 · United States
NIGMS NIH HHS · GM46226 · United States
NIGMS NIH HHS · R01 GM046226-11 · United States
NIGMS NIH HHS · R01 GM046226-12 · United States
NIGMS NIH HHS · R01 GM046226-10A1 · United States
NIGMS NIH HHS · R01 GM046226-13 · United States
NIGMS NIH HHS · R01 GM046226 · United States
NIGMS NIH HHS · R01 GM046226-13S1 · United States
External Links
PubMed source DOI: 10.2144/02335bm02 PMC Full Text View journal details
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