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PMID: 12451175 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 25 ·2002-12-10 ·Pages 16319-24

Iwai H, Masaoka N, Ishii T, Satoh S

Abstract

Intercellular attachment is an essential process in the morphogenesis of multicellular organisms. A unique mutant, nolac-H18 (nonorganogenic callus with loosely attached cells), generated by T-DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, lost the ability to form tight intercellular attachments and adventitious shoots. The gene tagged with T-DNA, named NpGUT1 (glucuronyltransferase 1), was similar to the gene for the catalytic domains of animal glucuronyltransferases and was expressed predominantly in shoot and root apical meristems. The transformation of NpGUT1 complemented the nolac-H18 mutation, and the expression of antisense NpGUT1 RNA produced crumbled shoots. The mutation caused defects in the glucuronic acid of rhamnogalacturonan II of pectin, which drastically reduced the formation of borate cross-linking of rhamnogalacturonan II. NpGUT1, which encodes a unique glucuronyltransferase, is a glycosyltransferase gene identified in pectin biosynthesis and is essential for intercellular attachment in plant meristems and tissues.

MeSH Terms
Amino Acid Sequence Carbohydrate Sequence Carbohydrates/analysis Cell Adhesion Cell Wall/chemistry Dimerization Genes, Recessive Glucuronosyltransferase/genetics,physiology Haploidy Meristem/cytology,metabolism Microscopy, Electron, Scanning Molecular Sequence Data Pectins/biosynthesis Plant Proteins/genetics,physiology Plant Shoots RNA, Antisense/pharmacology RNA, Messenger/biosynthesis RNA, Plant/biosynthesis Sequence Alignment Sequence Homology, Amino Acid Tobacco/enzymology
Chemicals
Carbohydrates Plant Proteins RNA, Antisense RNA, Messenger RNA, Plant Pectins galactosylgalactoylxylosylprotein 3-beta-glucuronosyltransferase Glucuronosyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Iwai Hiroaki
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Masaoka Nobutaka
Ishii Tadashi
Satoh Shinobu
References (28)
28 references, click to expand
  1. Selective trafficking of KNOTTED1 homeodomain protein and its mRNA through plasmodesmata.
    Science. 1995 Dec 22;270(5244):1980-3 PMID: 8533088
  2. Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE.
    Methods Enzymol. 1993;218:340-56 PMID: 7685466
  3. Cell-cell signaling in the shoot meristem.
    Curr Opin Plant Biol. 2001 Oct;4(5):441-6 PMID: 11597503
  4. The link between heparan sulfate and hereditary bone disease: finding a function for the EXT family of putative tumor suppressor proteins.
    J Clin Invest. 2001 Aug;108(4):511-6 PMID: 11518722
  5. Molecular analysis of cellulose biosynthesis in Arabidopsis.
    Science. 1998 Jan 30;279(5351):717-20 PMID: 9445479
  6. Borate-rhamnogalacturonan II bonding reinforced by Ca2+ retains pectic polysaccharides in higher-plant cell walls
    Plant Physiol. 1999 Jan;119(1):199-204 PMID: 9880361
  7. Transposition of the maize autonomous element Activator in transgenic Nicotiana plumbaginifolia plants.
    Mol Gen Genet. 1993 Apr;238(1-2):209-17 PMID: 8386800
  8. Pectic polysaccharide rhamnogalacturonan II is covalently linked to homogalacturonan.
    Phytochemistry. 2001 Jul;57(6):969-74 PMID: 11423143
  9. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking.
    Genomics. 1995 Feb 10;25(3):674-81 PMID: 7759102
  10. Structural characterization of the pectic polysaccharide rhamnogalacturonan II: evidence for the backbone location of the aceric acid-containing oligoglycosyl side chain.
    Carbohydr Res. 2000 Jun 30;326(4):277-94 PMID: 10890275
  11. Formation of rhamnogalacturonan II-borate dimer in pectin determines cell wall thickness of pumpkin tissue.
    Plant Physiol. 2001 Aug;126(4):1698-705 PMID: 11500567
  12. BORON IN PLANT STRUCTURE AND FUNCTION.
    Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:481-500 PMID: 15012243
  13. The plant cell wall polysaccharide rhamnogalacturonan II self-assembles into a covalently cross-linked dimer.
    J Biol Chem. 1999 May 7;274(19):13098-104 PMID: 10224062
  14. A lily stylar pectin is necessary for pollen tube adhesion to an in vitro stylar matrix.
    Plant Cell. 2000 Sep;12(9):1737-50 PMID: 11006344
  15. Pectin: cell biology and prospects for functional analysis.
    Plant Mol Biol. 2001 Sep;47(1-2):9-27 PMID: 11554482
  16. Pectins: structure, biosynthesis, and oligogalacturonide-related signaling.
    Phytochemistry. 2001 Jul;57(6):929-67 PMID: 11423142
  17. Requirement of borate cross-linking of cell wall rhamnogalacturonan II for Arabidopsis growth.
    Science. 2001 Oct 26;294(5543):846-9 PMID: 11679668
  18. Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development.
    Plant Cell. 1994 Jul;6(7):967-81 PMID: 8069107
  19. Isolation and structural characterization of beta-D-glucosyluronic acid and 4-O-methyl beta-D-glucosyluronic acid-containing oligosaccharides from the cell-wall pectic polysaccharide, rhamnogalacturonan I.
    Carbohydr Res. 1994 Jan 15;252:235-43 PMID: 8137363
  20. Plant glycosyltransferases.
    Curr Opin Plant Biol. 2001 Jun;4(3):219-24 PMID: 11312132
  21. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth.
    Plant J. 1993 Jan;3(1):1-30 PMID: 8401598
  22. Variations in the structure of neutral sugar chains in the pectic polysaccharides of morphologically different carrot calli and correlations with the size of cell clusters.
    Planta. 1996;198(4):634-9 PMID: 8727449
  23. Isolation and analysis of cell walls from plant material.
    Methods Biochem Anal. 1987;32:25-153 PMID: 3574138
  24. Rhamnogalacturonan-II, a pectic polysaccharide in the walls of growing plant cell, forms a dimer that is covalently cross-linked by a borate ester. In vitro conditions for the formation and hydrolysis of the dimer.
    J Biol Chem. 1996 Sep 13;271(37):22923-30 PMID: 8798473
  25. Alpha-glucosidase I is required for cellulose biosynthesis and morphogenesis in Arabidopsis.
    J Cell Biol. 2002 Mar 18;156(6):1003-13 PMID: 11901167
  26. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  27. Absence of arabinan in the side chains of the pectic polysaccharides strongly associated with cell walls of Nicotiana plumbaginifolia non-organogenic callus with loosely attached constituent cells.
    Planta. 2001 Oct;213(6):907-15 PMID: 11722127
  28. Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis.
    Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2262-7 PMID: 11226227
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.252530499
Published
2002-12-10
Epub
2002-00-25
Pages
16319-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC138609
Subset
IM
Databases
GENBANK
AB080676, AB080693, AB080694
Corrections
CommentIn
PMID: 12461188
External Links
PubMed source DOI: 10.1073/pnas.252530499 PMC Full Text View journal details
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