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PMID: 1245553 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effects of local anesthetics on cell morphology and membrane-associated cytoskeletal organization in BALB/3T3 cells.

The Journal of cell biology ·Vol. 68 ·No. 2 ·1976-02-00 ·Pages 395-402

Nicolson GL, Smith JR, Poste G

Abstract

Tertiary amine local anesthetics (dibucaine, tetracaine, procaine) reversibly affect the morphology of untransformed BALB/3T3 cells and the organization of membrane-associated cytoskeletal elements. In the presence of these drugs cells contract and become rounded in shape with the appearance of numerous surface "blebs." Electron microscope examination of anesthetic-treated cells revealed significant reductions in plasma membrane-associated microtubules and microfilaments and/or their plasma membrane attachment. The relationship of the findings on local anesthetic-induced changes in cellular cytoskeletal systems is discussed in relation to previous proposals on plasma membrane organization and control of cell surface receptor topography and mobility.

MeSH Terms
Cell Line Cell Membrane/drug effects,ultrastructure Cells, Cultured/drug effects,ultrastructure Dibucaine/pharmacology Dose-Response Relationship, Drug Microtubules/drug effects,ultrastructure Procaine/pharmacology Tetracaine/pharmacology
Chemicals
Tetracaine Procaine Dibucaine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nicolson G L
Smith J R
Poste G
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42 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.68.2.395
Published
1976-02-00
Pages
395-402
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109628
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.68.2.395 PMC Full Text View journal details
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