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PMID: 1245601 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The role and fate of rabbit and human transcobalamin II in the plasma transport of vitamin B12 in the rabbit.

The Journal of clinical investigation ·Vol. 57 ·No. 1 ·1976-01-00 ·Pages 27-38

Schneider RJ, Burger RL, Mehlman CS, Allen RH

Abstract

Previous studies have shown that plasma transcobalamin II (TCII) facilitates the cellular uptake of [57Co] vitamin B12 (B12) by a variety of tissues, but the lack of an intrinsic label on the protein moiety of the TCII-B12 complex has made it impossible to determine the role and fate of TCII during this process. We have labeled homogensous rabbit and human TCII with 125I-labeled N-succinimidyl-3-(4-hydroxyphenyl) propionate and have performed in vivo experiments in rabbits. When 125I-labeled rabbit TCII-[57Co] B12 and 131I-labeled bovine albumin were simultaneously injected intravenously, we observed that 125Iand 57Co were cleared from plasma at a faster rate (t1/2 = 1 1/2 h) than 131I and that 125I and 57Co were present in excess of 131I in the kidney, liver, spleen, heart, lung, and small intestine 1/2 h after injection. Later, 57Co remained in excess of 131I, but the ratio of 125I to 131I decreased progressively in all of these plasma and were rapidly excreted in the urine. After 1 h following injection, 57Co was present in excess of 125I in the plasma...

MeSH Terms
Animals Biological Transport Blood Proteins/physiology Extracellular Space/metabolism Humans Iodine Radioisotopes Male Protein Binding Rabbits Time Factors Transcobalamins/metabolism,physiology Vitamin B 12/blood
Chemicals
Blood Proteins Iodine Radioisotopes Transcobalamins Vitamin B 12
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schneider R J
Burger R L
Mehlman C S
Allen R H
References (26)
26 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108265
Published
1976-01-00
Pages
27-38
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC436621
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108265 PMC Full Text View journal details
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