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PMID: 12456661 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.

The EMBO journal ·Vol. 21 ·No. 23 ·2002-12-02 ·Pages 6549-59

Soppe WJ, Jasencakova Z, Houben A, Kakutani T, Meister A, Huang MS, Jacobsen SE, Schubert I, Fransz PF

Abstract

We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F(1) hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.

MeSH Terms
Arabidopsis/metabolism Arabidopsis Proteins DNA Methylation DNA-Binding Proteins/genetics Gene Silencing Heterochromatin/metabolism Histones/metabolism Mutation Transcription Factors/genetics
Chemicals
Arabidopsis Proteins DDM1 protein, Arabidopsis DNA-Binding Proteins Heterochromatin Histones Transcription Factors
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Soppe Wim J J
Department of Cytogenetics, Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
Jasencakova Zuzana
Houben Andreas
Kakutani Tetsuji
Meister Armin
Huang Michael S
Jacobsen Steven E
Schubert Ingo
Fransz Paul F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf657
Published
2002-12-02
Pages
6549-59
Language
English
Region
England
NLM ID
8208664
PMCID
PMC136960
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf657 PMC Full Text View journal details
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