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PMID: 12486013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The function of SECIS RNA in translational control of gene expression in Escherichia coli.

The EMBO journal ·Vol. 21 ·No. 24 ·2002-12-16 ·Pages 6925-34

Thanbichler M, Böck A

Abstract

The incorporation of selenocysteine into proteins is directed by specific UGA codons and mRNA secondary structures, designated SECIS elements. In bacteria, these elements are positioned within the reading frame of selenoprotein mRNAs immediately downstream of the triplet coding for selenocysteine, and they tether a complex of the selenocysteine-specific elongation factor SelB, GTP and selenocysteyl-tRNA(Sec) to the site of UGA decoding. A SECIS-like structure was identified in the 5' non-translated region of the selAB transcript, encoding selenocysteine synthase and SelB. It specifically binds to SelB and the formation of a SelB.GTP.selenocysteyl-tRNA(Sec) complex on the SECIS-like element represses expression of the downstream gene. This effect is abolished by mutations preventing formation of the complex. The regulatory pattern observed correlated with the levels of sel gene products. As quaternary complex formation on the SECIS-like element did not influence the transcription rate and only slightly reduced the level of selAB mRNA, it was concluded that the structure is involved in regulating translation initiation efficiency, thereby coupling selenocysteine biosynthesis to the availability of the trace element selenium.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Escherichia coli/metabolism Immunoblotting Lac Operon Models, Biological Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Plasmids/metabolism Protein Biosynthesis RNA/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Selenium/metabolism Single-Strand Specific DNA and RNA Endonucleases/pharmacology Time Factors Transcription, Genetic beta-Galactosidase/metabolism
Chemicals
Bacterial Proteins RNA, Messenger RNA-Binding Proteins SelB protein, Bacteria RNA Single-Strand Specific DNA and RNA Endonucleases beta-Galactosidase Selenium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thanbichler Martin
Department of Biology I, Microbiology, University of Munich, Maria-Ward-Strasse 1a, D-80638 Munich, Germany.
Böck August
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf673
Published
2002-12-16
Pages
6925-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC139081
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf673 PMC Full Text View journal details
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