Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.

Infection and immunity ·Vol. 13 ·No. 1 ·1976-01-00 ·Pages 133-9

Honda T, Taga S, Takeda T, Hasibuan MA, Takeda Y, Miwatani T

Abstract

Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was demonstrated by sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis and analytical ultracentrifugation. It was demonstrated that the thermostable direct hemolysin was identical to the lethal toxin and that it was the main, if not only, lethal toxin in the culture filtrate. The purified toxin had a lethal effect when injected into mice either intravenously or intraperitoneally. Its lethal effect was very rapid, a dose of 5 mug of toxin per mouse killing the animals within 1 min. The lethal activity was inhibited by a ganglioside mixture. Some physicochemical properties of the purified toxin are reported.

MeSH Terms

Animals Hemolysin Proteins/isolation & purification Hot Temperature Injections, Intravenous Mice Toxins, Biological/administration & dosage,analysis,isolation & purification Vibrio parahaemolyticus

Chemicals

Hemolysin Proteins Toxins, Biological

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Honda T

Taga S

Takeda T

Hasibuan M A

Takeda Y

Miwatani T

References (13)

13 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.13.1.133-139.1976

Published

1976-01-00

Pages

133-9

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC420587

Subset

External Links

PubMed source DOI: 10.1128/iai.13.1.133-139.1976 PMC Full Text View journal details

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Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.

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