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PMID: 12496208 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells.

Infection and immunity ·Vol. 71 ·No. 1 ·2003-01-00 ·Pages 541-5

Hassane DC, Lee RB, Pickett CL

Abstract

Cytolethal distending toxin (CDT) is a multisubunit protein found in various gram-negative bacterial pathogens of humans which is thought to cause cell death by direct DNA damage of host cells. We sought to determine if a cellular response to DNA damage could be detected by exogenous addition of the holotoxin. Exogenous addition of the Campylobacter jejuni 81-176 CDT to primary human fibroblasts resulted in formation of Rad50 foci, which are formed around double-stranded-DNA breaks. Moreover, such foci are formed in both proliferating and nonproliferating cells that are treated with C. jejuni CDT. Fibroblasts that were intoxicated and later stimulated to proliferate failed to divide and remained arrested in the G(1) phase of the cell cycle.

MeSH Terms
Acid Anhydride Hydrolases Bacterial Toxins/pharmacology Campylobacter jejuni/metabolism Cell Cycle/drug effects Cell Division/drug effects Cells, Cultured DNA Repair/drug effects DNA Repair Enzymes DNA-Binding Proteins/metabolism Fibroblasts Humans
Chemicals
Bacterial Toxins DNA-Binding Proteins cytolethal distending toxin Acid Anhydride Hydrolases Rad50 protein, human DNA Repair Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hassane Duane C
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky 40536, USA.
Lee Robert B
Pickett Carol L
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.71.1.541-545.2003
Published
2003-01-00
Pages
541-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC143155
Subset
IM
Grants
NIAID NIH HHS · AI48590 · United States
External Links
PubMed source DOI: 10.1128/IAI.71.1.541-545.2003 PMC Full Text View journal details
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