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PMID: 1250708 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromatin model calculations: Arrays of spherical nu bodies.

Nucleic acids research ·Vol. 3 ·No. 1 ·1976-01-00 ·Pages 89-100

Carlson RD, Olins DE

Abstract

Chromatin fibers consists of globular nucleohistone particles (designated nu bodies) along the length of the chromatin DNA with approximately 6-to7-fold compaction of the DNA within the nu bodies. We have calculated theoretical small-angle x-ray scattering curves and have compared these with experimental data in the literature. Several models predict maxima at the correct angles. The first maximum (approximately 110 degrees A) results from interparticle interference, while both the spatial arrangement and the structure factor the nu bodies can contribute to the additional small-angle maxima. These calculations suggest models which can account for the electron microscopic observation that chromatin is seen as either approximately 100-or approximately 200-to 250 degrees A-diameter fibers, depending on the solvent conditions. They also account for the limited orientability of the x-ray pattern from pulled chromatin fibers.

MeSH Terms
Binding Sites Chromatin/ultrastructure DNA Mathematics Microscopy, Electron Nucleic Acid Conformation X-Ray Diffraction
Chemicals
Chromatin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Carlson R D
Olins D E
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.1.89
Published
1976-01-00
Pages
89-100
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342880
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.1.89 PMC Full Text View journal details
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