Abstract
The differentiation of vegetative cells of Bacillus subtilis into spores involves asymmetric cell division, which precedes complete chromosome partitioning. The DNA translocase SpoIIIE is required to translocate the origin distal 70% of the chromosome from the larger mother cell into the smaller prespore, the two cells that result from the division. We have tested the effect of altering the time and location of SpoIIIE synthesis on spore formation. We have expressed the spoIIIE homologue from Sporosarcina ureae in B. subtilis under the control of different promoters. Expression from either a weak mother cell-specific (sigma(E)) promoter or a weak prespore-specific (sigma(F)) promoter partly complemented the sporulation defect of a spoIIIE36 mutant; however, expression from a strong prespore-specific (sigma(F)) promoter did not. DNA translocation from the mother cell to the prespore was assayed using spoIIQ-lacZ inserted at thrC; transcription of spoIIQ occurs only in the prespore. Translocation of thrC::spoIIQ-lacZ into the prespore occurred efficiently when spoIIIE(Su) was expressed from the weak sigma(E)- or sigma(F)-controlled promoters but not when it was expressed from the strong sigma(F)-controlled promoter. It is speculated that the mechanism directing SpoIIIE insertion into the septum in the correct orientation may accommodate slow postseptational, prespore-specific SpoIIIE synthesis but may be swamped by strong prespore-specific synthesis.
MeSH Terms
Bacillus subtilis/genetics,physiology
Bacterial Proteins/genetics,physiology
Biological Transport
DNA, Bacterial/metabolism
Gram-Positive Endospore-Forming Bacteria/enzymology
Promoter Regions, Genetic
Sigma Factor
Spores, Bacterial/physiology
Transcription Factors
Chemicals
Bacterial Proteins
DNA, Bacterial
Sigma Factor
Transcription Factors
spoIIR protein, Bacillus subtilis
spore-specific proteins, Bacillus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chary Vasant K
Department of Microbiology and Immunology, Temple University School of Medicine, 3400 N. Broad Street, Philadelphia, PA 19140, USA.
Piggot Patrick J
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