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PMID: 12538643 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

RhoA is required for cortical retraction and rigidity during mitotic cell rounding.

The Journal of cell biology ·Vol. 160 ·No. 2 ·2003-01-20 ·Pages 255-65

Maddox AS, Burridge K

Abstract

Mitotic cell rounding is the process of cell shape change in which a flat interphase cell becomes spherical at the onset of mitosis. Rearrangement of the actin cytoskeleton, de-adhesion, and an increase in cortical rigidity accompany mitotic cell rounding. The molecular mechanisms that contribute to this process have not been defined. We show that RhoA is required for cortical retraction but not de-adhesion during mitotic cell rounding. The mitotic increase in cortical rigidity also requires RhoA, suggesting that increases in cortical rigidity and cortical retraction are linked processes. Rho-kinase is also required for mitotic cortical retraction and rigidity, indicating that the effects of RhoA on cell rounding are mediated through this effector. Consistent with a role for RhoA during mitotic entry, RhoA activity is elevated in rounded, preanaphase mitotic cells. The activity of the RhoA inhibitor p190RhoGAP is decreased due to its serine/threonine phosphorylation at this time. Cumulatively, these results suggest that the mitotic increase in RhoA activity leads to rearrangements of the cortical actin cytoskeleton that promote cortical rigidity, resulting in mitotic cell rounding.

MeSH Terms
Actin Cytoskeleton/drug effects,metabolism,ultrastructure Anaphase/drug effects,physiology Cell Adhesion/drug effects,physiology Cell Size/drug effects,physiology Cytoskeleton/drug effects,metabolism,ultrastructure Enzyme Inhibitors/pharmacology Eukaryotic Cells/cytology,drug effects,metabolism HeLa Cells Humans Interphase/drug effects,physiology Intracellular Signaling Peptides and Proteins Mitosis/drug effects,physiology Protein Serine-Threonine Kinases/antagonists & inhibitors,metabolism rho-Associated Kinases rhoA GTP-Binding Protein/metabolism
Chemicals
Enzyme Inhibitors Intracellular Signaling Peptides and Proteins Protein Serine-Threonine Kinases rho-Associated Kinases rhoA GTP-Binding Protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maddox Amy Shaub
Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA. [email protected]
Burridge Keith
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200207130
Email
[email protected]
Published
2003-01-20
Epub
2003-00-21
Pages
255-65
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172639
Subset
IM
Grants
NIGMS NIH HHS · R01 GM029860 · United States
NIGMS NIH HHS · GM29860 · United States
External Links
PubMed source DOI: 10.1083/jcb.200207130 PMC Full Text View journal details
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