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PMID: 1254640 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.

The Journal of cell biology ·Vol. 69 ·No. 1 ·1976-04-00 ·Pages 144-58

Fertuck HC, Salpeter MM

Abstract

The distribution and quantitation of 125I-alpha-bungarotoxin (alpha-BTX) binding sites and thus acetylcholine receptor (AChR) were determined in mouse sternomastoid muscle by electron microscope autoradiography. We found that a valid criterion for receptor saturation at the neuromuscular junction was the complete elimination of neurally evoked tetanic muscle contractions, since, when such a criterion was used for the endpoint of toxin incubation, alpha-BTX was bound to approximately 90% of total available endplate sites. When, without implying localization, the presynaptic axonal membrane was used as a convenient reference structure, the concentration of alpha-BTX relative to this membrane was determined to be 46,000 +/- 27% sites/mum2.

MeSH Terms
Acetylcholine Animals Autoradiography Binding Sites Membranes/ultrastructure Mice Models, Biological Muscles/innervation,ultrastructure Neuromuscular Junction/metabolism,ultrastructure Receptors, Drug Synaptic Membranes/ultrastructure
Chemicals
Receptors, Drug Acetylcholine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fertuck H C
Salpeter M M
References (35)
35 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.69.1.144
Published
1976-04-00
Pages
144-58
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110977
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.69.1.144 PMC Full Text View journal details
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