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PMID: 1254644 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Carbamycholine prevents giant granule-formation in cultured fibroblasts from beige (Chediak-Higashi) mice.

The Journal of cell biology ·Vol. 69 ·No. 1 ·1976-04-00 ·Pages 205-10

Oliver JM, Krawiec JA, Berlin RD

Abstract

Primary embryonic fibroblasts isolated from beige (Chediak-Higashi) mice develop pathognomonic giant granules in vitro. Inclusion in the culture medium of carbamylcholine (carbachol) or phorbol myristate acetate (PMA) results in the generation of morphologically normally granules. Chediak-Higashi fibroblasts are highly susceptible to shape changes induced by colchicine. This abnormal property is also corrected by carbachol and PMA.

MeSH Terms
Atropine/pharmacology Carbachol/pharmacology Cells, Cultured Chediak-Higashi Syndrome/pathology Dose-Response Relationship, Drug Lysosomes/drug effects,ultrastructure Phorbols/pharmacology
Chemicals
Phorbols Atropine Carbachol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Oliver J M
Krawiec J A
Berlin R D
References (19)
19 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.69.1.205
Published
1976-04-00
Pages
205-10
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110978
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.69.1.205 PMC Full Text View journal details
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