[Studies on the complete enzymatic hydrolysis of steroid conjugates in urine (author's transl)].

Untersuchungen zur vollständigen enzymatischen Hydrolyse von Steroidkonjugaten im Harn

Zeitschrift fur klinische Chemie und klinische Biochemie ·Vol. 13 ·No. 4 ·1975-04-00 ·Pages 163-7

Graef V, Fuchs M

Abstract

The incubation of urine with beta-glucuronidase/arylsuphatase from Helix pomatia results in the incomplete hydrolysis of urinary steroid conjugates, because inhibitors of these enzymes are present in urine; The inhibitors can be separated from the steroid conjugates by chromatography of the urine on a column of Amberlite XAD-2. By this method a complete enzymatic hydrolysis of urinary steroid conjugates is possible in 24 hours at 37 degrees C or in 2 hours at 55 degrees C.

MeSH Terms

Androsterone/urine Arylsulfatases/antagonists & inhibitors Chromatography, Ion Exchange Chromatography, Thin Layer Dehydroepiandrosterone/urine Enzyme Inhibitors/urine Estriol/urine Etiocholanolone/urine Female Glucuronidase/antagonists & inhibitors Helix, Snails Humans Hydrolysis Pregnancy Pregnanediol/urine Pregnanetriol/urine Steroids/urine

Chemicals

Enzyme Inhibitors Steroids Pregnanetriol Dehydroepiandrosterone Etiocholanolone Androsterone Arylsulfatases Glucuronidase Estriol Pregnanediol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Graef V

Fuchs M

Article Info

Journal

Zeitschrift fur klinische Chemie und klinische Biochemie

Abbr.

Z Klin Chem Klin Biochem

ISSN

0044-2933

Published

1975-04-00

Pages

163-7

Language

ger

Region

Germany

NLM ID

7607250

Subset

External Links

PubMed source

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[Studies on the complete enzymatic hydrolysis of steroid conjugates in urine (author's transl)].

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