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PMID: 12551954 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dynactin is required for bidirectional organelle transport.

The Journal of cell biology ·Vol. 160 ·No. 3 ·2003-02-03 ·Pages 297-301

Deacon SW, Serpinskaya AS, Vaughan PS, Lopez Fanarraga M, Vernos I, Vaughan KT, Gelfand VI

Abstract

Kinesin II is a heterotrimeric plus end-directed microtubule motor responsible for the anterograde movement of organelles in various cell types. Despite substantial literature concerning the types of organelles that kinesin II transports, the question of how this motor associates with cargo organelles remains unanswered. To address this question, we have used Xenopus laevis melanophores as a model system. Through analysis of kinesin II-mediated melanosome motility, we have determined that the dynactin complex, known as an anchor for cytoplasmic dynein, also links kinesin II to organelles. Biochemical data demonstrates that the putative cargo-binding subunit of Xenopus kinesin II, Xenopus kinesin II-associated protein (XKAP), binds directly to the p150Glued subunit of dynactin. This interaction occurs through aa 530-793 of XKAP and aa 600-811 of p150Glued. These results reveal that dynactin is required for transport activity of microtubule motors of opposite polarity, cytoplasmic dynein and kinesin II, and may provide a new mechanism to coordinate their activities.

MeSH Terms
Animals Binding, Competitive/physiology Caenorhabditis elegans Proteins Calcium-Binding Proteins/metabolism Cells, Cultured Dynactin Complex Kinesins/metabolism Macromolecular Substances Melanophores/metabolism Melanosomes/metabolism Microtubule-Associated Proteins/metabolism Models, Biological Muscle Proteins/metabolism Organelles/metabolism Protein Binding/physiology Protein Transport/physiology Xenopus Proteins Xenopus laevis
Chemicals
Caenorhabditis elegans Proteins Calcium-Binding Proteins Dynactin Complex Macromolecular Substances Microtubule-Associated Proteins Muscle Proteins OSM-3 protein, C elegans XKLP3 protein, Xenopus Xenopus Proteins kinesin-II Kinesins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Deacon Sean W
Department of Cell and Structural Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801.
Serpinskaya Anna S
Vaughan Patricia S
Lopez Fanarraga Monica
Vernos Isabelle
Vaughan Kevin T
Gelfand Vladimir I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200210066
Published
2003-02-03
Epub
2003-00-27
Pages
297-301
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172679
Subset
IM
Grants
NIGMS NIH HHS · GM52111 · United States
NIGMS NIH HHS · R01 GM052111 · United States
NIGMS NIH HHS · T32 GM07283 · United States
NIGMS NIH HHS · R01 GM060560 · United States
NIGMS NIH HHS · T32 GM007283 · United States
NIGMS NIH HHS · GM60560 · United States
Corrections
CommentIn
PMID: 12566424
External Links
PubMed source DOI: 10.1083/jcb.200210066 PMC Full Text View journal details
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