Home LiteratureArticle Details
PMID: 1255852 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and properties of bacteriophage phi X 174 gene D product.

Journal of virology ·Vol. 17 ·No. 3 ·1976-03-00 ·Pages 1027-37

Farber MB

Abstract

Bacteriophage phi X 174 gene D product, a protein required for single-stranded DNA synthesis by the phage, has been purified to near homogeneity. The protein is very abundant; approximately 10(5) monomers are present per infected cell when lysis is delayed. The protein has a monomer molecular weight of 15,200 and is normally a tetramer; however, it can form very large aggregates at high concentrations. Amino acid analysis shows an excess of arginine over lysine and a relatively high number of nonpolar residues. The protein carries a net negative charge at neutral pH. The first eight amino acids of the protein sequence have been determined; they are Ser-Gln-Val-Thr-Glu-Gln-Arg-Val. The carboxy-terminal residue is methionine. The protein has not yet been shown to bind directly to any single-stranded DNA; it does not adsorb to denatured calf thymus DNA-cellulose.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Arginine/analysis Coliphages/analysis,metabolism DNA Viruses DNA, Single-Stranded/biosynthesis,metabolism DNA, Viral/biosynthesis,metabolism Genes Molecular Weight Peptides/analysis Protein Binding Protein Biosynthesis Viral Proteins/analysis,isolation & purification,metabolism
Chemicals
Amino Acids DNA, Single-Stranded DNA, Viral Peptides Viral Proteins Arginine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Farber M B
References (32)
32 references, click to expand
  1. A DNA-protein complex involved in bacteriophage phi chi 174 particle formation.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):3054-8 PMID: 4528401
  2. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 PMID: 4560696
  3. Gene A of X174. 1. Isolation and identification of its products.
    Virology. 1972 Nov;50(2):381-7 PMID: 4564522
  4. Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity.
    J Biol Chem. 1973 Jan 10;248(1):110-21 PMID: 4571224
  5. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53 PMID: 4598307
  6. Class of phi chi 174 mutants relatively deficient in synthesis of viral RNA.
    J Virol. 1974 Dec;14(6):1630-2 PMID: 4610194
  7. Role of the gene beta-product in bacteriophage phi-X174 development.
    J Mol Biol. 1974 Oct 15;89(1):1-16 PMID: 4613851
  8. Mechanism of head assembly and DNA encapsulation in Salmonella phage P22. II. Morphogenetic pathway.
    J Mol Biol. 1973 Nov 15;80(4):697-731 PMID: 4773027
  9. DNA replication: the rolling circle model.
    Cold Spring Harb Symp Quant Biol. 1968;33:473-84 PMID: 4891987
  10. Studies on phiX174 proteins. I. Phage-specific proteins synthesized after infection of Escherichia coli.
    J Mol Biol. 1969 Sep 28;44(3):377-86 PMID: 4899472
  11. The mechanism of replication of phi X174 single-stranded DNA. II. The role of viral proteins.
    J Mol Biol. 1971 Apr 28;57(2):159-75 PMID: 4931509
  12. Role of coliphage M13 gene 5 in single-stranded DNA production.
    J Mol Biol. 1971 Nov 14;61(3):489-501 PMID: 4943754
  13. The process of infection with bacteriophage phiX174. XXVII. Synthesis of a viral-specific chloramphenicol-resistant protein in phiX174-infected cells.
    J Mol Biol. 1969 Feb 14;39(3):655-68 PMID: 5357216
  14. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  15. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene.
    J Mol Biol. 1966 Jul;18(3):429-47 PMID: 5968177
  16. Spectroscopic determination of tryptophan and tyrosine in proteins.
    Biochemistry. 1967 Jul;6(7):1948-54 PMID: 6049437
  17. A protein sequenator.
    Eur J Biochem. 1967 Mar;1(1):80-91 PMID: 6059350
  18. Genetic Map of Bacteriophage phiX174.
    J Virol. 1971 May;7(5):549-58 PMID: 16789129
  19. Isolation and characterization of gene 5 protein of filamentous bacterial viruses.
    J Mol Biol. 1972 Jul 14;68(1):139-52 PMID: 4115107
  20. Properties of the isolated gene 5 protein of bacteriophage fd.
    J Mol Biol. 1972 Jul 14;68(1):125-38 PMID: 4559110
  21. A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41 PMID: 4566449
  22. Polypeptide bound to the host RNA polymerase is specified by T4 control gene 33.
    Nat New Biol. 1973 Aug 1;244(135):137-40 PMID: 4579464
  23. The amino acid sequence of a DNA binding protein, the gene 5 product of fd filamentous bacteriophage.
    FEBS Lett. 1974 Apr 1;40(2):290-2 PMID: 4605211
  24. Regulation of coliphage f1 single-stranded DNA synthesis by a DNA-binding protein.
    J Mol Biol. 1973 Aug 5;78(2):285-300 PMID: 4747632
  25. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant.
    J Mol Biol. 1967 Aug 28;28(1):87-94 PMID: 4860988
  26. Electrophoretic characterization of phiX174-specific proteins.
    J Mol Biol. 1969 Sep 28;44(3):501-16 PMID: 4899475
  27. Characterization and synthesis of phi X174 proteins in ultraviolet-irradiated and unirradiated cells.
    J Mol Biol. 1971 May 14;57(3):541-53 PMID: 4931681
  28. The process of infection with bacteriophage phi-X174. IV. Replication of the viral DNA in a synchronized infection.
    J Mol Biol. 1965 Jul;12(3):647-62 PMID: 5323481
  29. The process of infection with bacteriophage phi-X174. IX. Studies on the physiology of three phi-X174 temperature-sensitive mutants.
    J Mol Biol. 1966 Apr;16(2):374-86 PMID: 5954170
  30. Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines.
    Biochim Biophys Acta. 1966 Dec 28;130(2):538-40 PMID: 5972864
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  32. Direction of Translation and Size of Bacteriophage phiX174 Cistrons.
    J Virol. 1972 Jul;10(1):99-114 PMID: 16789133
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.17.3.1027-1037.1976
Published
1976-03-00
Pages
1027-37
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515501
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.17.3.1027-1037.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2