Reaction of HeLa cell methyl-labelled 28S ribosomal RNA with sodium bisulphite: a conformational probe for methylated sequences.

Nucleic acids research ·Vol. 3 ·No. 2 ·1976-02-00 ·Pages 431-40

Goddard JP, Maden BE

Abstract

The reaction of 14C methyl-labelled HeLa cell 28 S ribosomal RNA with sodium bisulphite was studied. Using conditions under which 30% of the total cytidine residues were de-aminated to uridine, the reactivities of individual cytidine residues near particular methylation sites differed widely; some underwent almost quantitative reaction, some showed intermediate reactivity and others were almost inert. The possible value of this method as a conformational probe for ribosomal RNA is discussed.

MeSH Terms

Base Sequence Binding Sites HeLa Cells Methylation Oligoribonucleotides/analysis RNA, Ribosomal Sulfites

Chemicals

Oligoribonucleotides RNA, Ribosomal Sulfites

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goddard J P

Maden B E

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.2.431

Published

1976-02-00

Pages

431-40

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342913

Subset

External Links

PubMed source DOI: 10.1093/nar/3.2.431 PMC Full Text View journal details

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Reaction of HeLa cell methyl-labelled 28S ribosomal RNA with sodium bisulphite: a conformational probe for methylated sequences.

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